التفاصيل البيبلوغرافية
| العنوان: |
The Use of the Neoglycolipid-Based Oligosaccharide Microarray System in the Diagnosis of Endometriosis – Preliminary Study. |
| المؤلفون: |
Wojtyla, Cezary, Tołwiński, Ignacy, Laudański, Piotr |
| المصدر: |
Journal of Inflammation Research; Feb2024, Vol. 17, p899-908, 10p |
| مصطلحات موضوعية: |
ENDOMETRIOSIS, ASCITIC fluids, DNA microarrays, BIOMATERIALS, LAPAROSCOPIC surgery, DIAGNOSIS, SERODIAGNOSIS |
| مصطلحات جغرافية: |
POLAND |
| مستخلص: |
Endometriosis presents diagnostic challenges, and there is a need for developing novel biomarkers with satisfactory specificity and sensitivity. Glycomics, exploring glycosylation changes in glycoproteins, offers potential solutions. The aim of this study was to analyze the carbohydrate-binding properties of IgG and IgM antibodies in the plasma and peritoneal fluid samples and to identify any differences in the presence and the specificities of anti-carbohydrate antibodies in the endometriosis patient and the controls. Methods: Multicenter study was conducted in Poland between 2018 and 2019. Plasma and peritoneal fluid samples were collected from women undergoing laparoscopic surgery. Endometriosis patients (n=8) and controls (n=8), matched for cycle phase and disease stage, were selected. The neoglycolipid-based oligosaccharide microarray system was used to investigate IgG and IgM antibody binding properties to glycan-related probes in biological materials. Results: In peritoneal fluid samples, IgM binding to the following probes was significantly higher in endometriosis: GSC-915-4 (new), LNFP-I, NeuAcα-(6')LNnO (F1), B-like decaosylceramide, log10(GM1-penta), and log10(GSC-915-5). In a control group higher IgG binding to log10(Orsay-5-AO) was observed. In plasma samples, endometriosis showed higher IgG binding to log10(NeuAcα-(6')LNnO (F1)) and lower IgG binding to Gal2GlcNAc(1-3)-AO. After Benjamin–Hochberg correction, differences were not significant. Effect sizes highlighted some glycan probes in both plasma and peritoneal fluid. Strong correlations were observed among binding to certain glycan probes. Conclusion: This preliminary study suggests glycomics' potential contribution to endometriosis diagnosis and understanding of its pathophysiology. Neoglycolipid-based microarrays hold promise for non-invasive endometriosis diagnostic tools. Further investigations with larger cohorts are warranted to validate these findings and explore potential correlations with antibody levels in plasma and peritoneal fluid. Glycomics emerges as a valuable diagnostic asset in endometriosis research. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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