A novel EGFR-targeted gene delivery system based on complexes self-assembled by EGF, DNA, and activated PAMAM dendrimers
| العنوان: | A novel EGFR-targeted gene delivery system based on complexes self-assembled by EGF, DNA, and activated PAMAM dendrimers |
|---|---|
| المؤلفون: | Mingshu Ma, Lan Wang, Xiaoning Zhang, Nan Liu, Zhe Yin, Yanli Hao |
| المصدر: | International Journal of Nanomedicine, Vol 2012, Iss default, Pp 4625-4635 (2012) International Journal of Nanomedicine |
| بيانات النشر: | Dove Medical Press, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Biodistribution, Dendrimers, Medicine (General), Cell Survival, Genetic enhancement, activated dendriplexes, Green Fluorescent Proteins, Biophysics, Pharmaceutical Science, Bioengineering, Biology, Gene delivery, Transfection, Biomaterials, Mice, R5-920, Drug Stability, Epidermal growth factor, International Journal of Nanomedicine, Dendrimer, Drug Discovery, self-assembled EGF modification, Animals, Humans, Particle Size, transfection efficiency, EGFR targeting, Original Research, Cell Proliferation, Drug Carriers, Mice, Inbred BALB C, Spectroscopy, Near-Infrared, Epidermal Growth Factor, Organic Chemistry, HEK 293 cells, General Medicine, DNA, Molecular biology, Cell biology, ErbB Receptors, HEK293 Cells, Cancer cell, MCF-7 Cells, Female |
| الوصف: | Zhe Yin*, Nan Liu*, Mingshu Ma, Lan Wang, Yanli Hao, Xiaoning Zhang Laboratory of Pharmaceutics, School of Medicine, Tsinghua University, Beijing, China*These authors contributed equally to this workAbstract: Epidermal growth factor receptor (EGFR)-targeted gene delivery is a promising approach in gene therapy against EGFR-positive cancer. In addition, macromolecules, such as polyamidoamine (PAMAM) dendrimers, are potential nonviral gene carriers in this therapy because of their biocompatibility and modifiable features. To achieve the goal of selectively enhancing the transfection efficiency in EGFR-positive cancer cells, the researchers developed chemical approaches of EGF-dendrimer conjugate, which were effective but complicated. Studies on liposomes reveal that self-assembly is another effective but simpler approach in EGF modification. Moreover, properly activated PAMAM dendrimers exhibit higher transfection efficiency, but little research has been done on its ligand-modification. In this study, we developed and characterized a novel gene-delivery system based on activated EGF-dendriplexes, which is formed via self-assembly by EGF and complexes prepared by activated PAMAM dendrimer and plasmid DNA. Such complexes exhibit desired features compared to nonmodified or nonactivated dendriplexes in vitro, including selective enhancement of transfection efficiency in EGFR-positive cells, decreased cytotoxicity, and low agonist effect. In vivo experimentation shows their EGFR-positive tumor targeted biodistribution and increased transfection efficiency at EGFR-positive tumors. Our results demonstrated that activated EGF-dendriplexes are safe and effective carriers for delivering gene drugs to EGFR-positive cells, which makes these complexes a promising targeted nonviral gene-delivery system for auxiliary cancer therapy.Keywords: activated dendriplexes, transfection efficiency, EGFR targeting, self-assembled EGF modification |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 1178-2013 1176-9114 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9207912739e54d38686702eaab8f56d7Test http://www.dovepress.com/a-novel-egfr-targeted-gene-delivery-system-based-on-complexes-self-ass-a10817Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9207912739e54d38686702eaab8f56d7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 11782013 11769114 |
|---|