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1دورية أكاديمية
المصدر: International Journal of General Medicine, Vol Volume 16, Pp 2671-2678 (2023)
مصطلحات موضوعية: mmp7, colorectal cancer, folfox4 chemotherapy, chemotherapy-resistant, Medicine (General), R5-920
الوصف: Yeting Zhou, Leiming Wang, Fei Zhou Xuzhou Medical University Jiangsu Key Laboratory of Tumor Biotherapy, Shuyang People’s Hospital, Suqian, 221000, People’s Republic of ChinaCorrespondence: Leiming Wang; Fei Zhou, Xuzhou Medical University Jiangsu Key Laboratory of Tumor Biotherapy, Shuyang People’s Hospital, Shuyang, Suqian, 221000, People’s Republic of China, Email wangleiming89@21cn.com; zhoufei67a@126.comObjective: Various studies have shown an association between the anti-cancer drug 5-fluorouracil and matrix metalloproteinase 7 (MMP7). The expression of MMP7 in the serum of colorectal cancer patients, as well as their sensitivity to chemotherapy, were examined using the FOLFOX4 chemotherapy treatment.Methods: Serum samples were taken from 216 colorectal cancer patients who had undergone four cycles of gemcitabine and cisplatin treatment. The sera of 216 healthy persons were used as controls. MMP7 levels in the serum were measured by ELISA. Demographic and survival data were collected.Results: MMP7 levels were not associated with sex, age, peritoneal dissemination, liver metastasis, lymph node metastasis, lymphatic invasion, or venous invasion in CRC patients, but were associated with histological grade, tumor size, TNM stage, and depth of tumor invasion. Patients’ serum MMP7 expression reduced after treatment. MMP7 expression was significantly lower chemotherapy-sensitive patients compared with chemotherapy-resistant patients. Elevated MMP7 expression was associated with worse prognosis and chemotherapy-sensitive patients had markedly better overall survival compared with chemotherapy-resistant patients.Conclusion: MMP7 expression was potentially associated with the development of colorectal cancer and elevated levels were associated with chemoresistance in CRC patients. Serum MMP7 levels can be used to screen for drug resistance during FOLFOX4 chemotherapy treatment.Keywords: MMP7, colorectal cancer, FOLFOX4 chemotherapy, chemotherapy-resistant
وصف الملف: electronic resource
العلاقة: https://www.dovepress.com/clinical-significance-ofTest-mmp7-levels-in-colorectal-cancer-patients-rec-peer-reviewed-fulltext-article-IJGM; https://doaj.org/toc/1178-7074Test
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2دورية أكاديمية
المصدر: OncoTargets and Therapy, Vol Volume 12, Pp 10963-10973 (2019)
مصطلحات موضوعية: glioma, bcar4, cerna, mir-2276, mmp7, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
الوصف: Zhifeng Wang, Longlong Wang, Zan Liang, Yanguo Xi Department of Neurosurgery, Cangzhou Central Hospital, Cangzhou, Hebei Province, People’s Republic of ChinaCorrespondence: Zhifeng Wang; Yanguo XiDepartment of Neurosurgery, Cangzhou Central Hospital, No. 16 Xinhuaxi Road, Cangzhou City, Hebei 061000, People’s Republic of ChinaTel +86 317 207 5627Email zhifengwang_czns@aliyun.comObjective: Long non-coding RNA breast cancer anti-estrogen resistance 4 (BCAR4) has been recognized as a proto-oncogene in various malignancies. It has been reported to be highly expressed and promote cell proliferation in glioma. However, its additional roles in gliomagenesis remain largely unclear. This research intends to investigate the impact and internal molecular mechanism of BCAR4 on glioma cell growth, invasion and tumorigenesis.Methods: BCAR4 expression was examined by qPCR in 30 cases of graded glioma specimens and 7 glioblastoma (GBM) cell lines compared with respective controls. Its potential prognostic value was evaluated by Kaplan–Meier survival analysis. The biological roles of BCAR4 in gliomagenesis were verified by CCK-8, transwell and intracranial xenograft assays successively. qPCR and RNA pull-down assays were applied to study the relationship between BCAR4 and miR-2276. Then, qPCR, Western blot and luciferase reporter assays were used to validate the targeting of matrix metallopeptidase 7 (MMP7) by miR-2276 and the regulation of MMP7 by BCAR4. Finally, MMP7 was restored in BCAR4-silenced GBM cells and the rescue effects were determined by CCK-8 and transwell assays.Results: BCAR4 expression was increased in glioma tissues and GBM cell lines, and its high expression positively correlated with advanced grades and worse prognosis. Functional assays verified that knockdown of BCAR4-inhibited cell growth and invasion in vitro, and impaired tumor formation in vivo. Mechanistically, we found that BCAR4 could act as a competing endogenous RNA (ceRNA) by targeting miR-2276 to upregulate MMP7 expression. Importantly, MMP7 restoration effectively rescued the inhibitory modulations on GBM cell growth and invasion caused by BCAR4 knockdown.Conclusion: Our findings identified the essential roles of the BCAR4/miR-2276/MMP7 axis in gliomagenesis and provided novel insights on glioma therapy.Keywords: glioma, BCAR4, ceRNA, miR-2276, MMP7
وصف الملف: electronic resource
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3دورية أكاديمية
المصدر: Cancer Management and Research, Vol Volume 11, Pp 547-559 (2019)
مصطلحات موضوعية: osteosarcoma, prognosis, metastasis, Notch3, Hes1, MMP7, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
الوصف: Xue-Feng Tang,1 Ya Cao,1 Dong-Bin Peng,1 Guo-Sheng Zhao,2 Ying Zeng,1,3 Zi-Ran Gao,1 Yang-Fan Lv,1 Qiao-Nan Guo1 1Department of Pathology, Xinqiao Hospital, Army Medical University, Chongqing 400037, China; 2Department of Orthopedic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; 3Department of Pathology, Daping Hospital, Army Medical University, Chongqing 400037, China Background: Notch signaling abnormalities are associated with the development of various tumors, including hematopoietic and epithelium-derived tumors. However, the role of Notch signaling in tumors originating from mesenchymal cells is unclear. The effect of Notch3 expression on the prognosis of osteosarcoma and its role and mechanism in osteosarcoma cells have never been reported. Materials and methods: In this study, we performed a clinicopathological analysis of 70 cases of osteosarcoma, with primary focus on survival. Osteosarcoma cell lines MTH and U2OS were used. After knockdown of Notch3 by lentiviral transfection and siRNA, the cell cycle, cell viability, and wound healing capacity were assessed. Subsequently, the Transwell assay was performed, and the expression levels of hairy and enhancer of split-1 (Hes1) and matrix metalloproteinase 7 (MMP7) were detected by RT-PCR and Western blot assay. The expression of MMP7 was also detected after knockdown of Hes1. Animal experiments were performed by injecting the cell lines MTH of Notch3 knockdown into mice tail veins and comparing the development of lung metastasis with the control group. Results: Comparison of survival curves showed that Notch3 expression significantly impacts patient survival. Additionally, multivariate analysis revealed that Notch3 is an independent prognostic factor for osteosarcoma. In in vivo experiments, osteosarcoma-associated pulmonary metastasis in nude mice was reduced after Notch3 silencing. The expression of downstream effector molecule, Hes1, and that of the invasion and metastasis-associated proteolytic enzyme, MMP7, were reduced, and MMP7 was further decreased by Hes1 knockdown in in vitro experiments. Conclusion: Notch3 is a prognostic factor for osteosarcoma and might regulate its invasion and metastasis through the downstream target gene Hes1 and effector MMP7. Keywords: osteosarcoma, prognosis, metastasis, Notch3, Hes1, MMP7
وصف الملف: electronic resource
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