Cytoskeleton Architecture of C6 Rat Glioma Cell Subclones Whole Mount Electron Microscopy and Immunogold Labeling
| العنوان: | Cytoskeleton Architecture of C6 Rat Glioma Cell Subclones Whole Mount Electron Microscopy and Immunogold Labeling |
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| المؤلفون: | Bohn W, Etzrodt D, Roland Foisner, Wiche G, Traub P |
| المصدر: | Scanning Microscopy Scopus-Elsevier Europe PubMed Central |
| بيانات النشر: | DigitalCommons@USU, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | intermediate filaments, plectin, immunogold labeling, cytoskeleton, Glioma, macromolecular substances, whole mount, Immunohistochemistry, Actins, Clone Cells, Rats, Cytoskeletal Proteins, Microscopy, Electron, vimentin, Intermediate Filament Proteins, Tumor Cells, Cultured, Animals, Connecting filaments, Biology |
| الوصف: | Whole mount electron microscopy of extracted cells combined with immunogold labeling techniques can be used to characterize the cytoskeletal architecture of cultured cells. As shown with subclones of the C6 rat glioma cell line, heavy metal shadowing was suitable for getting basic information concerning the arrangement of the various filament types within the networks. Pure carbon shadowing combined with immunogold double labeling proved to be optimal to identify linkages between filaments, to localize filament associated proteins and to follow the arrangement of filaments in dense arrays such as lamellipodiae and cell margins. Thin connecting filaments which interact with actin as well as with vimentin filaments and can be labeled with antibodies to the intermediate filament associated protein plectin may play a major role in the structural organization of the cytoskeleton of these cells. |
| وصف الملف: | application/pdf |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::0d97189ffb6c4052cc5b47e65f18e5b0Test https://digitalcommons.usu.edu/context/microscopy/article/1631/viewcontent/WDCcenterscan1996BohnEtzrodtFoisner_CytoskeletonArchitectureC6.pdfTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....0d97189ffb6c4052cc5b47e65f18e5b0 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |