دورية أكاديمية
The Catabolite Control Protein E (CcpE) Affects Virulence Determinant Production and Pathogenesis of Staphylococcus aureus
| العنوان: | The Catabolite Control Protein E (CcpE) Affects Virulence Determinant Production and Pathogenesis of Staphylococcus aureus |
|---|---|
| المؤلفون: | Hartmann, Torsten, Baronian, Grégory, Nippe, Nadine, Voss, Meike, Schulthess, Bettina, Wolz, Christiane, Eisenbeis, Janina, Schmidt-Hohagen, Kerstin, Gaupp, Rosmarie, Sunderkötter, Cord, Beisswenger, Christoph, Bals, Robert, Somerville, Greg A., Herrmann, Mathias, Molle, Virginie, Bischoff, Markus |
| المصدر: | Papers in Veterinary and Biomedical Science |
| بيانات النشر: | DigitalCommons@University of Nebraska - Lincoln |
| سنة النشر: | 2014 |
| المجموعة: | University of Nebraska-Lincoln: DigitalCommons@UNL |
| مصطلحات موضوعية: | Bacterial Pathogenesis, Staphylococcus aureus (S. aureus), Transcription Regulation, Tricarboxylic Acid Cycle (TCA Cycle) (Krebs Cycle), Virulence Factor, Animal Diseases, Cell and Developmental Biology, Life Sciences, Medicine and Health Sciences, Respiratory Tract Diseases, Veterinary Infectious Diseases, Veterinary Medicine, Veterinary Microbiology and Immunobiology, Veterinary Pathology and Pathobiology |
| الوصف: | Carbon metabolism and virulence determinant production are often linked in pathogenic bacteria, and several regulatory elements have been reported to mediate this linkage in Staphylococcus aureus. Previously, we described a novel protein, catabolite control protein E (CcpE) that functions as a regulator of the tricarboxylic acid cycle. Here we demonstrate that CcpE also regulates virulence determinant biosynthesis and pathogenesis. Specifically, deletion of ccpE in S. aureus strain Newman revealed that CcpE affects transcription of virulence factors such as capA, the first gene in the capsule biosynthetic operon; hla, encoding α-toxin; and psmα, encoding the phenol-soluble modulin cluster α. Electrophoretic mobility shift assays demonstrated that CcpE binds to the hla promoter. Mice challenged with S. aureus strain Newman or its isogenic ΔccpE derivative revealed increased disease severity in the ΔccpE mutant using two animal models; an acute lung infection model and a skin infection model. Complementation of the mutant with the ccpE wild-type allele restored all phenotypes, demonstrating that CcpE is negative regulator of virulence in S. aureus. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://digitalcommons.unl.edu/vetscipapers/159Test; https://digitalcommons.unl.edu/context/vetscipapers/article/1162/viewcontent/Somerville_JBC_2014_Catabolite_Control_Protein__DC_VERSION__2.pdfTest |
| الإتاحة: | https://digitalcommons.unl.edu/vetscipapers/159Test https://digitalcommons.unl.edu/context/vetscipapers/article/1162/viewcontent/Somerville_JBC_2014_Catabolite_Control_Protein__DC_VERSION__2.pdfTest |
| رقم الانضمام: | edsbas.C349AEBF |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |