التفاصيل البيبلوغرافية
| العنوان: |
Cross-serotype protection against group A Streptococcal infections induced by immunization with SPy_2191 |
| المؤلفون: |
Sanduja, Pooja, Gupta, Manish, Somani, Vikas Kumar, Yadav, Vikas, Dua, Meenakshi, Hanski, Emanuel, Sharma, Abhinay, Bhatnagar, Rakesh, Johri, Atul Kumar |
| المصدر: |
Open Access Publications |
| بيانات النشر: |
Digital Commons@Becker |
| سنة النشر: |
2020 |
| المجموعة: |
Washington University School of Medicine: Digital Commons@Becker |
| مصطلحات موضوعية: |
Animals, Bacterial Adhesion, Bacterial Proteins, Cell Line, Cloning, Molecular, Cross Protection, Disease Models, Animal, Female, Humans, Immunogenicity, Vaccine, Mice, Neutralization Tests, Recombinant Proteins, Serogroup, Streptococcal Infections, Streptococcal Vaccines, Streptococcus pyogenes, Vaccines, Synthetic |
| الوصف: |
Group A Streptococcus (GAS) infection causes a range of diseases, but vaccine development is hampered by the high number of serotypes. Here, using reverse vaccinology the authors identify SPy_2191 as a cross-protective vaccine candidate. From 18 initially identified surface proteins, only SPy_2191 is conserved, surface-exposed and inhibits both GAS adhesion and invasion. SPy_2191 immunization in mice generates bactericidal antibodies resulting in opsonophagocytic killing of prevalent and invasive GAS serotypes of different geographical regions, including M1 and M49 (India), M3.1 (Israel), M1 (UK) and M1 (USA). Resident splenocytes show higher interferon-γ and tumor necrosis factor-α secretion upon antigen re-stimulation, suggesting activation of cell-mediated immunity. SPy_2191 immunization significantly reduces streptococcal load in the organs and confers ~76-92% protection upon challenge with invasive GAS serotypes. Further, it significantly suppresses GAS pharyngeal colonization in mice mucosal infection model. Our findings suggest that SPy_2191 can act as a universal vaccine candidate against GAS infections. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
https://digitalcommons.wustl.edu/open_access_pubs/11964Test; https://digitalcommons.wustl.edu/context/open_access_pubs/article/12949/viewcontent/s41467_020_17299_x.pdfTest; https://digitalcommons.wustl.edu/context/open_access_pubs/article/12949/filename/0/type/additional/viewcontent/41467_2020_17299_MOESM1_ESM.pdfTest; https://digitalcommons.wustl.edu/context/open_access_pubs/article/12949/filename/1/type/additional/viewcontent/41467_2020_17299_MOESM2_ESM.pdfTest; https://digitalcommons.wustl.edu/context/open_access_pubs/article/12949/filename/2/type/additional/viewcontent/41467_2020_17299_MOESM3_ESM.pdfTest; https://digitalcommons.wustl.edu/context/open_access_pubs/article/12949/filename/3/type/additional/viewcontent/41467_2020_17299_MOESM4_ESM.xlsxTest |
| DOI: |
10.1038/s41467-020-17299-x |
| الإتاحة: |
https://doi.org/10.1038/s41467-020-17299-xTest
https://digitalcommons.wustl.edu/open_access_pubs/11964Test
https://digitalcommons.wustl.edu/context/open_access_pubs/article/12949/viewcontent/s41467_020_17299_x.pdfTest
https://digitalcommons.wustl.edu/context/open_access_pubs/article/12949/filename/0/type/additional/viewcontent/41467_2020_17299_MOESM1_ESM.pdfTest
https://digitalcommons.wustl.edu/context/open_access_pubs/article/12949/filename/1/type/additional/viewcontent/41467_2020_17299_MOESM2_ESM.pdfTest
https://digitalcommons.wustl.edu/context/open_access_pubs/article/12949/filename/2/type/additional/viewcontent/41467_2020_17299_MOESM3_ESM.pdfTest
https://digitalcommons.wustl.edu/context/open_access_pubs/article/12949/filename/3/type/additional/viewcontent/41467_2020_17299_MOESM4_ESM.xlsxTest |
| رقم الانضمام: |
edsbas.E1907511 |
| قاعدة البيانات: |
BASE |
