دورية أكاديمية
Pro-inflammatory biomarkers during experimental gingivitis in patients with type 1 diabetes mellitus: A proof-of-concept study
| العنوان: | Pro-inflammatory biomarkers during experimental gingivitis in patients with type 1 diabetes mellitus: A proof-of-concept study |
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| المؤلفون: | Salvi, GE, Giannobile, WV, Lee, A, Rutger Persson, G, Franco, LM, Braun, TM, Lang, NP |
| بيانات النشر: | //www.blackwellpublishing.com/journals/CPE Denmark |
| سنة النشر: | 2010 |
| المجموعة: | University of Hong Kong: HKU Scholars Hub |
| مصطلحات موضوعية: | Periodontal Index, Nucleic Acid Hybridization, Matrix Metalloproteinase 9 - analysis, Matrix Metalloproteinase 8 - analysis, Interleukin-8 - analysis, Gingival Crevicular Fluid - chemistry, Gingivitis - metabolism, Case-Control Studies, Cohort Studies, Adolescent, Adult, Bacteria - classification, Biofilms, Biological Markers - analysis, Dental Plaque - microbiology, Humans, Interleukin-1beta - analysis, Inflammation Mediators - analysis, Dental Plaque Index, Diabetes Mellitus, Type 1 - complications - metabolism, Young Adult, Prospective Studies, Follow-Up Studies |
| الوصف: | Aim: To compare gingival crevicular fluid (GCF) biomarker levels and microbial distribution in plaque biofilm (SP) samples for subjects with type 1 diabetes (T1DM) versus healthy subjects without diabetes during experimental gingivitis (EG). Materials and Methods: A total of nine T1DM patients and nine healthy controls of age and gender similar to the T1DM patients were monitored for 35 days during EG. Hygiene practices were stopped for 3 weeks, and GCF, SP, plaque index (PI) and gingival index were determined. IL-1β, IL-8, MMP-8 and MMP-9 were quantified by enzyme-linked immunosorbent assay, and SP samples were assessed by DNA-DNA hybridization for a panel of 40 subgingival microbial species. Results: IL-1β levels in T1DM patients were elevated compared with healthy individuals, and showed differences between groups at 7-21 days while healthy patients showed IL-1β increases from baseline to 14-21 days (p<0.05). Differences were observed in MMP-9 levels between patients with and without T1DM at 7-14 days (p<0.05). Orange complex species and PI measurements displayed a superior correlation with biomarker levels when compared with other complexes or clinical measurements during EG. Conclusions: The mean GCF biomarker levels for IL-1β and MMP-8 were most significantly elevated in T1DM subjects compared with healthy individuals during EG, not resulting from differences in the mean PI or microbial composition. © 2009 John Wiley & Sons A/S. ; link_to_subscribed_fulltext |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0303-6979 |
| العلاقة: | Journal of Clinical Periodontology; http://www.scopus.com/mlt/select.url?eid=2-s2.0-72549107943&selection=ref&src=s&origin=recordpageTest; Journal Of Clinical Periodontology, 2010, v. 37 n. 1, p. 9-16; 6466146; 16; 169087; WOS:000272771700002; http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0303-6979&volume=37&spage=9&epage=16&date=2010&atitle=Pro-inflammatory+biomarkers+during+experimental+gingivitis+in+patients+with+type+1+diabetes+mellitus:+a+proof-of-concept+studyTest; eid_2-s2.0-72549107943; http://hdl.handle.net/10722/66053Test; 37 |
| DOI: | 10.1111/j.1600-051X.2009.01500.x |
| الإتاحة: | https://doi.org/10.1111/j.1600-051X.2009.01500.xTest http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0303-6979&volume=37&spage=9&epage=16&date=2010&atitle=Pro-inflammatory+biomarkers+during+experimental+gingivitis+in+patients+with+type+1+diabetes+mellitus:+a+proof-of-concept+studyTest http://hdl.handle.net/10722/66053Test |
| رقم الانضمام: | edsbas.1D1DED08 |
| قاعدة البيانات: | BASE |
| تدمد: | 03036979 |
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| DOI: | 10.1111/j.1600-051X.2009.01500.x |