MiR-1225-5p acts as tumor suppressor in glioblastoma via targeting FNDC3B
| العنوان: | MiR-1225-5p acts as tumor suppressor in glioblastoma via targeting FNDC3B |
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| المؤلفون: | Cui Zhang, Chuan-Hui Wang, Shuai Cheng, Liang-Yan Wang, Peng Zhang, Guo-Hua Wang |
| المصدر: | Open Medicine Open Medicine, Vol 15, Iss 1, Pp 872-881 (2020) |
| بيانات النشر: | De Gruyter, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Poor prognosis, FNDC3B, Genome, law.invention, target, 03 medical and health sciences, 0302 clinical medicine, Western blot, law, Glioma, Cancer genome, medicine, miR-1225-5p, Reporter gene, regulate, medicine.diagnostic_test, business.industry, glioblastoma, General Medicine, medicine.disease, 030104 developmental biology, 030220 oncology & carcinogenesis, Cancer research, Medicine, Suppressor, business, Glioblastoma, Research Article |
| الوصف: | This study attempted to research the molecular mechanism underlying the inhibitory role of miR-1225-5p in the malignant progression of glioblastoma. Bioinformatics analyses based on the gene expression omnibus (GEO) and Chinese glioma genome atlas (CGGA) databases showed that miR-1225-5p, as a favorable prognostic factor, was expressed at low levels in glioblastoma, and its expression was also related to WHO grade and age. The subsequent CCK-8 assay indicated that miR-1225-5p might prevent the malignant progression of glioblastoma, which was represented by that miR-1225-5p mimic reduced the viability of glioblastoma cells. Then, we predicted that FNDC3B might be a potential target gene of miR-1225-5p, and it was negatively correlated with the level of miR-1225-5p, which were confirmed by dual-luciferase reporter assay, qRT-PCR and western blot assays. Moreover, based on the analyses of the cancer genome atlas (TCGA), Oncomine and CGGA databases, FNDC3B was enriched in glioblastoma and high expression of FNDC3B led to poor prognosis. Finally, CCK8 and transwell experiments showed that the ability of miR-1225-5p to inhibit glioblastoma cell viability, invasion and migration was at least partially achieved by targeting FNDC3B. In general, these results revealed that the miR-1225-5p/FNDC3B axis contributes to inhibiting the malignant phenotype of glioblastoma cells, which lays a foundation for molecular diagnosis and treatment of glioblastoma. |
| اللغة: | English |
| تدمد: | 2391-5463 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::694daca186fc0f4cb1999177256f5faeTest http://europepmc.org/articles/PMC7712056Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....694daca186fc0f4cb1999177256f5fae |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23915463 |
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