دورية أكاديمية
Role of Cyclic Di-GMP and Exopolysaccharide in Type IV Pilus Dynamics
| العنوان: | Role of Cyclic Di-GMP and Exopolysaccharide in Type IV Pilus Dynamics |
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| المؤلفون: | Ribbe, Jan, Baker, Amy E, Euler, Sebastian, O'Toole, George A, Maier, Berenike |
| المصدر: | Dartmouth Scholarship |
| بيانات النشر: | Dartmouth Digital Commons |
| سنة النشر: | 2017 |
| المجموعة: | Dartmouth Digital Commons (Dartmouth College) |
| مصطلحات موضوعية: | pseudomonas aeruginosa, biofilm, cyclic di-gmp, exopolysaccharide, molecular motor, pili, bacterial secretion systems, cyclic gmp, escherichia coli proteins, fimbriae, bacterial, gene deletion, gene expression regulation, enzymologic, movement, oxygen, phosphoric diester hydrolases, phosphorus-oxygen lyases, polysaccharides, pseudomonas, Medicine and Health Sciences |
| الوصف: | For Pseudomonas aeruginosa, levels of cyclic di-GMP (c-di-GMP) govern the transition from the planktonic state to biofilm formation. Type IV pili (T4P) are crucial determinants of biofilm structure and dynamics, but it is unknown how levels of c-di-GMP affect pilus dynamics. Here, we scrutinized how c-di-GMP affects molecular motor properties and adhesive behavior of T4P. By means of retraction, T4P generated forces of ∼30 pN. Deletion mutants in the proteins with known roles in biofilm formation, swarming motility, and exopolysaccharide (EPS) production (specifically, the diguanylate cyclases sadC and roeA or the c-di-GMP phosphodiesterase bifA) showed only modest effects on velocity or force of T4P retraction. At high levels of c-di-GMP, the production of exopolysaccharides, particularly of Pel, is upregulated. We found that Pel production strongly enhances T4P-mediated surface adhesion of P. aeruginosa, suggesting that T4P-matrix interactions may be involved in biofilm formation by P. aeruginosa. Finally, our data support the previously proposed model of slingshot-like “twitching” motility of P. aeruginosa. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://digitalcommons.dartmouth.edu/facoa/3176Test; https://digitalcommons.dartmouth.edu/context/facoa/article/4165/viewcontent/ptpmcrender.fcgiTest |
| DOI: | 10.1128/JB.00859-16 |
| الإتاحة: | https://doi.org/10.1128/JB.00859-16Test https://digitalcommons.dartmouth.edu/facoa/3176Test https://digitalcommons.dartmouth.edu/context/facoa/article/4165/viewcontent/ptpmcrender.fcgiTest |
| رقم الانضمام: | edsbas.D25E7FF7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/JB.00859-16 |
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