A hederagenin saponin isolated from Clematis ganpiniana induces apoptosis in breast cancer cells via the mitochondrial pathway
| العنوان: | A hederagenin saponin isolated from Clematis ganpiniana induces apoptosis in breast cancer cells via the mitochondrial pathway |
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| المؤلفون: | Xi Sun, Liang Shi, Tian‑Song Xia, Lei Zhu, Xiao-Xia Li, Xu-Jie Zhou, Lin Cheng, Qiang Ding, Jing Wu |
| المصدر: | Oncology Letters |
| بيانات النشر: | D.A. Spandidos, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Clematis, Cancer Research, mechanism, Pharmacology, Mitochondrion, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Breast cancer, breast cancer, Botany, medicine, Cytotoxic T cell, clematis hederagenin saponin, skin and connective tissue diseases, biology, Oncogene, apoptosis, Cancer, Articles, medicine.disease, biology.organism_classification, mitochondria, Hederagenin, 030104 developmental biology, Oncology, chemistry, Apoptosis, 030220 oncology & carcinogenesis |
| الوصف: | Natural plants offer a treasure trove of resources for anticancer drug development. Clematis are widely used in Traditional Chinese Medicine. However, studies on the active substances in Clematis are scarce. In the present study, four monomer compounds were successfully extracted from this species and their inhibitory effects on the growth of breast cancer cells were investigated using bioactivity tests. Among them, Clematis hederagenin saponin (CHS) belongs to the class of triterpenoid saponins. CHS showed cytotoxic effects on breast cancer cells in a dose- and time-dependent manner. The compound also induced apoptosis in breast cancer cells in a time-dependent manner. Further investigation into the underlying mechanisms of apoptosis induction in breast cancer cells showed that the compound significantly reduced mitochondrial Apaf-1 and cytochrome c proteins in breast cancer cells. In addition, it upregulated the activities of caspase-3 and -9. In conclusion, CHS induced apoptosis in breast cancer cells through regulation of the mitochondrial apoptosis pathway. The results suggest that the hederagenin saponin extracted from Clematis ganpiniana offers great potential as a novel anti-breast cancer drug. |
| اللغة: | English |
| تدمد: | 1792-1082 1792-1074 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d965514b6bf02dba14ba1d8dc0862ce9Test http://europepmc.org/articles/PMC5774383Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d965514b6bf02dba14ba1d8dc0862ce9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921082 17921074 |
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