Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis
| العنوان: | Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis |
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| المؤلفون: | Chuan-Yun Qian, Ming-wei Liu, Hui-Hui Li, Yun-Hui Wang |
| المصدر: | International Journal of Molecular Medicine |
| بيانات النشر: | D.A. Spandidos, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Male, Pathology, Gene Expression, Vascular permeability, Pharmacology, sepsis, Rats, Sprague-Dawley, oxidative stress, Cecum, Lung, medicine.diagnostic_test, Reverse Transcriptase Polymerase Chain Reaction, Intracellular Signaling Peptides and Proteins, General Medicine, Articles, Lung Injury, Intercellular Adhesion Molecule-1, Up-Regulation, interleukin-1 receptor-associated kinase 1, medicine.anatomical_structure, Interleukin-1 Receptor-Associated Kinases, Cytokines, Tumor necrosis factor alpha, medicine.symptom, medicine.medical_specialty, Blotting, Western, Inflammation, Enzyme-Linked Immunosorbent Assay, Punctures, Biology, Lung injury, Sepsis, Capillary Permeability, Xuebijing, von Willebrand Factor, Genetics, medicine, Animals, TNF Receptor-Associated Factor 6, TOLLIP, Transcription Factor RelA, Membrane Proteins, Toll-interacting protein, medicine.disease, lung permeability, rats, Toll-Like Receptor 4, Bronchoalveolar lavage, Drugs, Chinese Herbal, Phytotherapy |
| الوصف: | Xuebijing (XBJ) is a type of traditional Tibetan medicine, and previous pharmacological studies have shown that the ethanol extract is derived from Chuanxiong, Chishao, Danshen and Honghua. Chuanxiong, Chishao, Danshen and Honghua possesses potent anti-inflammatory activity, and has been used in the treatment of inflammatory infectious diseases. In the present study, we investigated the effects of XBJ on pulmonary permeability and lung injury in cecal ligation and puncture (CLP)-induced sepsis in rats. A CLP sepsis model was established for the control and treatment groups, respectively. Approximately 2 h prior to surgery, an amount of 100 mg/kg XBJ injection was administered to the treatment group. Reverse transcription polymerase chain reaction (PT-PCR) and western blot analysis were used to examine the expression of Toll-interacting protein (Tollip), interleukin-1 receptor-associated kinase 1 (IRAK1), Toll-like receptor 4 (TLR4), nuclear factor-κB65 (NF-κB65) and TNF receptor-associated factor 6 (TRAF6) in lung tissue. ELISA was applied to detect changes of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 (IL-1), interleukin-4 (IL-4) and interleukin-10 (IL-10) levels in bronchoalveolar lavage (BAL) fluid, and intercellular adhesion molecule 1 (ICAM-1) and von Willebrand factor (vWF) in serum. The number of neutrophils, albumin and total cells in the BAL fluid were measured. For histological analysis, hematoxylin and eosin (H&E) stains were evaluated. Lung permeability, the wet/dry weight ratio (W/D) and the lung pathology score were determined following the induction of ALI by CLP for 24 h. The results demonstrated that XBJ upregulated Tollip expression and blocked the activity of IRAK1, TLR4, NF-κβ65 and TRAF6. Additionally, the number of neutrophils and total cells were significantly decreased in the XBJ group compared to that in the control group. Lung permeability, the wet/dry weight ratio (W/D) and the lung pathology score were significantly decreased in the XBJ group. The histological results also demonstrated the attenuation effect of XBJ on CLP-induced lung inflammation. The results of the present study indicated that XBJ has a significantly reduced CLP-induced lung permeability by upregulating Tollip expression. The protective effects of XBJ suggest its therapeutic potential in CLP-induced acute lung injury treatment. |
| اللغة: | English |
| تدمد: | 1791-244X 1107-3756 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::438deeabfa656c5f864dbeba81c9445bTest http://europepmc.org/articles/PMC4214342Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....438deeabfa656c5f864dbeba81c9445b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1791244X 11073756 |
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