Three-dimensional hydrogel is suitable for targeted investigation of amoeboid migration of glioma cells
| العنوان: | Three-dimensional hydrogel is suitable for targeted investigation of amoeboid migration of glioma cells |
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| المؤلفون: | Haiwen Ma, Yang Xie, Li Yi, Peidong Liu, Wei Wang, Xuejun Yang, Luqing Tong, Shengping Yu, Long Hai, Zhennan Tao, Tao Li, Chen Zhang, Yubao Huang |
| المصدر: | Molecular Medicine Reports |
| بيانات النشر: | D.A. Spandidos, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, rac1 GTP-Binding Protein, Cancer Research, RHOA, Cell Culture Techniques, Motility, Gene Expression, RAC1, Biology, Biochemistry, Hydrogel, Polyethylene Glycol Dimethacrylate, Focal adhesion, 03 medical and health sciences, 0302 clinical medicine, amoeboid migration, Cell Movement, Cell Line, Tumor, Genetics, Tumor Cells, Cultured, three-dimensional, Humans, Molecular Biology, rho-Associated Kinases, targeted investigation, Mesenchymal stem cell, Cell migration, Articles, Glioma, Cell cycle, Prognosis, Cell biology, 030104 developmental biology, Oncology, Cell culture, 030220 oncology & carcinogenesis, Cancer research, biology.protein, Molecular Medicine, hydrogel, rhoA GTP-Binding Protein |
| الوصف: | Glioblastoma (GBM) invasion and migration are key biological behaviors leading to refractoriness to current therapies and infiltration into the non-tumor brain parenchyma. GBM cell migration is strongly dependent on tumor architecture in vivo, which is absent in traditional two-dimensional (2D) monolayer culture. The present study applied a three-dimensional (3D) hydrogel model to rebuild the tumor architecture in vitro. Treatment with NSC23766, a specific inhibitor of Ras-related C3 botulinum toxin substrate 1 (Rac1), inhibited the mesenchymal invasiveness however triggered the amoeboid motility called mesenchymal-amoeboid transition (MAT). Notably, NSC23766 stimulated U87 GBM cell migration in the 3D hydrogel. However, this compound inhibited cell motility in 2D monolayer culture without tumor architecture for MAT, suggesting the advantage of 3D hydrogel to investigate tumor cell invasion. Due to the inverse interaction of Rac1 and Ras homolog family member A (RhoA) signaling in the transition between mesenchymal and amoeboid morphology, simultaneous treatment of NSC23766 and Y27632 (selective Rho associated coiled-coil containing protein kinase 1 inhibitor), abolished U87 GBM cell migration through inhibiting MAT and amoeboid-mesenchymal transition. In addition, Y27632 induced integrin expression which gave rise to the focal adhesion to facilitate the mesenchymal invasion. The results of the present study demonstrated that the 3D hydrogel was a preferable model in vitro to study tumor cell invasion and migration. The combined inhibition of Rac1 and RhoA signaling would be a promising strategy to suppress GBM invasion. |
| اللغة: | English |
| تدمد: | 1791-3004 1791-2997 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf27e0698dee3880ec0f278747154e76Test http://europepmc.org/articles/PMC5780134Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cf27e0698dee3880ec0f278747154e76 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17913004 17912997 |
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