Fatty acid synthase-positive hepatocytes and subsequent steatosis in rat livers by irinotecan
| العنوان: | Fatty acid synthase-positive hepatocytes and subsequent steatosis in rat livers by irinotecan |
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| المؤلفون: | Satoko Morohashi, Takuya Miura, Hiroshi Kijima, Kenichi Hakamada, Shigeki Tsuchida, Takeyuki Sawano, Toshiyuki Yamada, Daisuke Kudo, Naoki Nanashima, Feng Mao Hui, Takeshi Shimizu |
| المصدر: | Oncology Reports |
| بيانات النشر: | D.A. Spandidos, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, Cancer Research, medicine.medical_specialty, medicine.medical_treatment, Intraperitoneal injection, H&E stain, Kupffer cell, liver progenitor cell, Irinotecan, Real-Time Polymerase Chain Reaction, Rats, Sprague-Dawley, chemistry.chemical_compound, Internal medicine, medicine, Animals, Progenitor cell, Oligonucleotide Array Sequence Analysis, fatty acid synthase, biology, Cholesterol, Fatty liver, General Medicine, Articles, medicine.disease, chemotherapy-associated steatohepatitis, Antineoplastic Agents, Phytogenic, Immunohistochemistry, Rats, Fatty Liver, Fatty acid synthase, Disease Models, Animal, Endocrinology, Oncology, chemistry, Liver, biology.protein, Hepatocytes, Camptothecin, Steatosis, Fatty Acid Synthases, Carbonic anhydrase 3 |
| الوصف: | Using a rat model, we investigated factors contributing to the pathogenesis of irinotecan-associated fatty liver disease. Male Sprague-Dawley rats were administered 200 mg/kg irinotecan by intraperitoneal injection on days 1-4, but not on days 5-7. This schedule was repeated 3 times. Rats were sacrificed 4, 18 and 25 days after the last injection, and liver steatosis was evaluated by hematoxylin and eosin (H&E) staining, microarray analysis and immunohistochemistry. Panacinar intrahepatocyte vacuoles were absent on days 4 and 25, but present on day 18, and this alteration was more prominent around the bile ducts than the central veins. Microarray analysis showed that the expression of genes involved in the synthesis of cholesterol and fatty acids was upregulated on day 4. Immunohistochemistry detected fatty acid synthase (Fasn)-strongly positive hepatocytes as well as the activation of liver progenitor cells on day 4, whereas intracellular vacuoles were evident in carbonic anhydrase 3 (CA3)-positive hepatocytes on day 18. Thus, irinotecan-induced liver steatosis was preceded by Fasn-strongly-positive hepatocytes and liver progenitor cell activation. The magnitude of the decrease in the number of Fasn-strongly positive hepatocytes between days 4 and 18 was similar to that of the increase in the number of CA3-positive hepatocytes accompanying vacuoles. |
| اللغة: | English |
| تدمد: | 1791-2431 1021-335X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f15d4927bb833214df69d89df4eb2fcdTest http://europepmc.org/articles/PMC4391592Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f15d4927bb833214df69d89df4eb2fcd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912431 1021335X |
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