Grape pomace extract exerts antioxidant effects through an increase in GCS levels and GST activity in muscle and endothelial cells
| العنوان: | Grape pomace extract exerts antioxidant effects through an increase in GCS levels and GST activity in muscle and endothelial cells |
|---|---|
| المؤلفون: | Alexios L. Skaltsounis, Christina Karapouliou, Aristidis Tsatsakis, Anastasia Housmekeridou, Demetrios Kouretas, Nektarios Aligiannis, Demetrios A. Spandidos, Dimitrios Stagos, Nikolaos Goutzourelas, Efthalia Kerasioti |
| المصدر: | International Journal of Molecular Medicine |
| بيانات النشر: | D.A. Spandidos, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Antioxidant, medicine.medical_treatment, Glutamate-Cysteine Ligase, Pharmacology, medicine.disease_cause, Antioxidants, Cell Line, Superoxide dismutase, chemistry.chemical_compound, Mice, Superoxide Dismutase-1, Genetics, medicine, Myocyte, grape pomace extract, Animals, Humans, Vitis, Glutathione Transferase, Muscle Cells, biology, Plant Extracts, Superoxide Dismutase, Skeletal muscle, Endothelial Cells, General Medicine, Glutathione, Articles, Catalase, Heme oxygenase, Enzyme Activation, Oxidative Stress, medicine.anatomical_structure, Biochemistry, chemistry, biology.protein, Oxidative stress, Heme Oxygenase-1 |
| الوصف: | In a previous study, we demonstrated that a grape pomace extract (GPE) exerted antioxidant activity in endothelial (EA.hy926) and muscle (C2C12) cells through an increase in glutathione (GSH) levels. In the present study, in order to elucidate the mechanisms responsible for the antioxidant activity of GPE, its effects on the expression of critical antioxidant enzymes, such as catalase (CAT), superoxide dismutase (SOD)1, heme oxygenase 1 (HO-1) and gamma-glutamylcysteine synthetase (GCS) were assessed in EA.hy926 and C2C12 cells. Moreover, the effects of GPE on CAT, SOD and glutathione S-transferase (GST) enzymatic activity were evaluated. For this purpose, the C2C12 and EA.hy926 cells were treated with GPE at low and non-cytotoxic concentrations (2.5 and 10 µg/ml for the C2C12 cells; 0.068 and 0.250 µg/ml for the EA.hy926 cells) for 3, 6, 12, 18 and 24 h. Following incubation, enzymatic expression and activity were assessed. The results revealed that treatment with GPE significantly increased GCS levels and GST activity in both the C2C12 and EA.hy926 cells. However, GPE significantly decreased CAT levels and activity, but only in the muscle cells, while it had no effect on CAT levels and activity in the endothelial cells. Moreover, treatment with GPE had no effect on HO-1 and SOD expression and activity in both cell lines. Therefore, the present results provide further evidence of the crucial role of GSH systems in the antioxidant effects exerted by GPE. Thus, GPE may prove to be effective for use as a food supplement for the treatment of oxidative stress-induced pathological conditions of the cardiovascular and skeletal muscle systems, particularly those associated with low GSH levels. |
| اللغة: | English |
| تدمد: | 1791-244X 1107-3756 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ecc2032368e6fce0721ed2bf94db3a2Test http://europepmc.org/articles/PMC4501638Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2ecc2032368e6fce0721ed2bf94db3a2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1791244X 11073756 |
|---|