Dehydrocorydaline promotes myogenic differentiation via p38 MAPK activation
| العنوان: | Dehydrocorydaline promotes myogenic differentiation via p38 MAPK activation |
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| المؤلفون: | Jong-Sun Kang, Yong Kee Kim, Miran Yoo, Dong-Wan Seo, Sang-Jin Lee, Jae-Ha Ryu, Gyu-Un Bae, Nam-In Baek |
| المصدر: | Molecular Medicine Reports |
| بيانات النشر: | D.A. Spandidos, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, MAPK/ERK pathway, Cancer Research, endocrine system, myoblast differentiation, p38 mitogen-activated protein kinases, Biology, p38 MAPK, MyoD, Muscle Development, Biochemistry, p38 Mitogen-Activated Protein Kinases, Cell Line, dehydrocorydaline, Myoblasts, 03 medical and health sciences, Mice, MyoD Protein, Alkaloids, Myosin, Genetics, Myocyte, Animals, Molecular Biology, Myogenin, Articles, musculoskeletal system, Molecular biology, Cell biology, Enzyme Activation, 030104 developmental biology, Oncology, Corydalis, Corydalis tuber, Molecular Medicine, C2C12, tissues, Drugs, Chinese Herbal |
| الوصف: | Muscle regeneration is a coordinated process that involves proliferation and differentiation of muscle progenitor cells. Activation of MyoD is a key event in myogenic differentiation, which is regulated by p38 mitogen-activated protein kinases (MAPK). In a screen of natural compounds for the enhancement of MyoD activity, dehydrocorydaline (DHC) from the Corydalis tuber was identified. Treatment of C2C12 myoblasts with DHC increased the expression levels of muscle-specific proteins, including MyoD, myogenin and myosin heavy chain. In addition, C2C12 myoblasts exhibited enhanced multinucleated myotube formation without any cytotoxicity. Treatment with DHC elevated p38 MAPK activation and the interaction of MyoD with an E protein, which is likely to result in activation of MyoD and promotion of myoblast differentiation. Furthermore, defects in differentiation-induced p38 MAPK activation and myoblast differentiation induced by depletion of the promyogenic receptor protein Cdo in C2C12 myoblasts were restored by DHC treatment. In conclusion, these results indicated that DHC stimulates p38 MAPK activation, which can enhance heterodimerization of MyoD and E proteins, thus resulting in MyoD activation and myoblast differentiation. These findings suggested that DHC may be considered a potential therapeutic compound for the improvement of muscle stem cell regenerative capacity in injured muscle. |
| اللغة: | English |
| تدمد: | 1791-3004 1791-2997 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3be3042c43bfa77c100169e31a0481faTest http://europepmc.org/articles/PMC5042734Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3be3042c43bfa77c100169e31a0481fa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17913004 17912997 |
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