دورية أكاديمية
TLR agonists activate HPV11 E7-pulsed DCs to promote a specific T cell response in a murine model
| العنوان: | TLR agonists activate HPV11 E7-pulsed DCs to promote a specific T cell response in a murine model |
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| المؤلفون: | X.H. Mao, X.Z. Chen, W.W. Zhang, J.Y. Wang, L.F. Liu, Q. Zhou, K.J. Zhu, H. Cheng |
| المصدر: | Veterinární Medicína, Vol 56, Iss 12, Pp 602-611 (2011) |
| بيانات النشر: | Czech Academy of Agricultural Sciences, 2011. |
| سنة النشر: | 2011 |
| المجموعة: | LCC:Veterinary medicine |
| مصطلحات موضوعية: | hpv - human papillomavirus, tlr - toll-like receptor, dc - dendritic cell, Veterinary medicine, SF600-1100 |
| الوصف: | Some TLR agonists may up-regulate the activation of dendritic cells caused by viral antigenic peptides and antigen-specific cytotoxic T lymphocytes, which are crucial in HPV vaccine development. We investigated the ability of three TLR agonists, imiquimod, PIC and CpG, to stimulate the maturation of murine BM-DCs loaded with HPV11E7 CTL epitopes, and the subsequent effect on HPV-specific T cell responses and tumour protection in a C57BL/6 mouse model. We found that TLR agonists, mostly PIC and imiquimod, stimulated the maturation of BM-DCs pulsed with HPV11E7 CTL epitope peptide. In combination with the epitope peptide, the TLR agonists CPG and PIC augmented epitope-specific Th1 cytokine production in vivo, while imiquimod and CPG, but not PIC, enhanced Th1 cytokine production in vitro. However, we failed to observe in vivo CTL cytotoxicity and anti-tumour protection upon TLR ligation in our mouse model. Our results demonstrate that TLR agonists activate HPV11E7 CTL epitope pulsed BM-DCs to promote specific Th1 immunity in C57BL/6 mouse model, indicating the promise of TLR agonists as adjuvants for HPV epitope/DC-based multifaceted vaccines against HPV infections such as condyloma accuminatum. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0375-8427 1805-9392 |
| العلاقة: | https://vetmed.agriculturejournals.cz/artkey/vet-201112-0005_tlr-agonists-activate-hpv11-e7-pulsed-dcs-to-promote-a-specific-t-cell-response-in-a-murine-model.phpTest; https://doaj.org/toc/0375-8427Test; https://doaj.org/toc/1805-9392Test |
| DOI: | 10.17221/4438-VETMED |
| الوصول الحر: | https://doaj.org/article/040e9e0eb9d74799a17a105b6f157abfTest |
| رقم الانضمام: | edsdoj.040e9e0eb9d74799a17a105b6f157abf |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 03758427 18059392 |
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| DOI: | 10.17221/4438-VETMED |