Molecular mechanisms of ceftazidime resistance in Pseudomonas aeruginosa isolates from canine and human infections
| العنوان: | Molecular mechanisms of ceftazidime resistance in Pseudomonas aeruginosa isolates from canine and human infections |
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| المؤلفون: | Hen-Wei Wei, S. J. Du, H. C. Kuo, Shao-Kuang Chang, Andrew Chang-Young Fei, Chiung-Hsiang Cheng |
| المصدر: | Veterinární medicína. 55:172-182 |
| بيانات النشر: | Czech Academy of Agricultural Sciences, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | 0301 basic medicine, General Veterinary, medicine.drug_class, Pseudomonas aeruginosa, medicine.medical_treatment, 030106 microbiology, Antibiotics, Cephalosporin, Ceftazidime, Drug resistance, biochemical phenomena, metabolism, and nutrition, Biology, bacterial infections and mycoses, medicine.disease_cause, Microbiology, Multiple drug resistance, 03 medical and health sciences, medicine, Beta-lactamase, Efflux, medicine.drug |
| الوصف: | Sixty-six clinical P. aeruginosa isolates, 17 obtained from canine otitis specimens and 49 received from human patients with bloodstream infections, were collected between February 2007 and January 2008. The minimal inhibitory concentrations (MICs) of the antimicrobial agents of these isolates were determined. Multidrug resistance was common, with 23 (34.8%) isolates found to be ceftazidime resistant. To explore the mechanisms of ceftazidime resistance, PCR analyses were performed to detect drug-resistance genes. The prevalence rate of Ambler class A, B, and D β-lactamase genes were obtained, with bla TEM-1 100%, bla PSE-1 100%, bla OXA-2 96.2%, bla SHV-18 91.3%, bla OXA-17 78.3%, bla VIM-3 26.1%, bla OXA-10 21.7% and bla SHV-1 8.7%. An efflux inhibition assay with the PAβN compound was conducted. The ceftazidime resistance isolates were also tested by RT-qPCR to deter- mine the mRNA expression levels of the oprM and ampC genes. Five (21.7%) of the ceftazidime resistance iso- lates appeared to overactivate the OprM efflux system. The ampD, ampE, and ampR genes and the ampC-ampR intergenic region were subsequently amplified and sequenced. Five (21.7%) of the ceftazidime resistance isolates from humans and canines had a point mutation in AmpR (Asp135-Asn, n = 3; Als194-Ser, n = 2), which induces AmpC overproduction from 10- to 80-fold. T his study first reported ceftazidime resistance in P. aeruginosa from canine otitis specimens, which are closely related to ESBLs (50%), including the overproduction of AmpC (25%) and the OprM efflux system (25%). The ESBLs (100%) played an important role in all ceftazidime resistance iso- lates from humans, and either AmpC (21.1%) or OprM (21.1%) might be overexpressed within the same isolate. A human patient isolate (H307B) showed simultaneous expression of ESBLs, the OprM efflux system, and AmpC overproduction. |
| تدمد: | 1805-9392 0375-8427 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::06e1c0b8a93f109b91718768fa48eb3fTest https://doi.org/10.17221/64/2010-vetmedTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........06e1c0b8a93f109b91718768fa48eb3f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18059392 03758427 |
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