Genomic structure and expression of the porcine ACTC1 gene
| العنوان: | Genomic structure and expression of the porcine ACTC1 gene |
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| المؤلفون: | Aleš Knoll, Antonín Stratil, Jaroslav Čítek, Mario Van Poucke, Kateřina Zadinová, Pavel Horak, Luc Peelman, Roman Stupka, Michaela Nesvadbová, Věra Dvořáková |
| المصدر: | Czech Journal of Animal Science. 63:371-378 |
| بيانات النشر: | Czech Academy of Agricultural Sciences, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Bacterial artificial chromosome, Real-time polymerase chain reaction, cDNA library, Complementary DNA, Intron, Coding region, Animal Science and Zoology, Single-nucleotide polymorphism, Biology, Gene, Molecular biology |
| الوصف: | A partial cDNA (similar to 1200 bp) of the porcine ACTC1 gene was identified in the subtracted foetal hind limb muscle cDNA library (44 days of gestation; using m. biceps femoris cDNA as the driver). Using specific polymerase chain reaction (PCR) primers, a bacterial artificial chromosome (BAC) clone containing the genomic ACTC1 gene was identified and the gene was sequenced. Specific PCR primers designed from the BAC and cDNA sequences were used for amplification and comparative sequencing of ACTC1 of Pietrain and Meishan pigs. The gene is approximately 5.4 kb in length, is composed of 7 exons, and has a coding sequence containing 1134 bp. The gene was mapped using the INRA-Minnesota porcine radiation hybrid (IMpRH) panel to chromosome 1, with SW65 as the closest marker (41 cR; LOD = 7.73). Differences were observed in tissue-specific expression of ACTC1 that was studied by transcription profiling in 28 porcine tissues. Developmental differences in muscle and heart were analysed by real-time quantitative PCR (RT-qPCR). Two single nucleotide polymorphisms (SNPs) were found in intron 1. One adequately informative SNP (FM212567.1:g.901C>G) was genotyped by PCR-restriction fragment length polymorphism, and allele frequencies in eight pig breeds were calculated. |
| تدمد: | 1805-9309 1212-1819 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::f20730e2fc307cdf4a78772aedd7ab0aTest https://doi.org/10.17221/34/2018-cjasTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........f20730e2fc307cdf4a78772aedd7ab0a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18059309 12121819 |
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