The influence of sample freezing at -80°C for 2-12 weeks on glycated haemoglobin (HbA1c) concentration assayed by HPLC method on Bio-Rad D-10© auto-analyzer
| العنوان: | The influence of sample freezing at -80°C for 2-12 weeks on glycated haemoglobin (HbA1c) concentration assayed by HPLC method on Bio-Rad D-10© auto-analyzer |
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| المؤلفون: | Katarzyna Bergmann, Grazyna Sypniewska |
| المصدر: | Biochemia Medica Volume 26 Issue 3 |
| بيانات النشر: | Croatian Society for Medical Biochemistry and Laboratory Medicine, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adult, Spectrum analyzer, high performance liquid chromatography, Clinical Biochemistry, 030209 endocrinology & metabolism, High-performance liquid chromatography, 03 medical and health sciences, sample storage, 0302 clinical medicine, Freezing, preanalytical phase, glycated haemoglobin, Humans, Hplc method, diabetes, Glycated haemoglobin, Chromatography, High Pressure Liquid, Aged, Whole blood, Aged, 80 and over, Glycated Hemoglobin, Chromatography, Chemistry, Biochemistry (medical), Significant difference, Healthy subjects, Middle Aged, Original Papers, Diabetes Mellitus, Type 1, Diabetes Mellitus, Type 2, Case-Control Studies, 030220 oncology & carcinogenesis, Positive bias |
| الوصف: | Introduction The aim of the study was to evaluate the effect of a single freeze/thaw cycle on HbA1c concentrations measured by commercially available HPLC method. Materials and methods Study included 128 whole blood samples collected from diabetic patients (N = 60) and healthy volunteers (N = 68). HbA1c concentrations were measured in fresh blood samples. Then samples were frozen at - 80 °C for up to 12 weeks. HbA1c was assayed by ion-exchange HPLC method on Bio-Rad D-10® analyzer. Variables were compared using Wilcoxon and ANOVA Kruskal-Wallis tests. Bias between HbA1c measured in fresh and frozen samples was calculated. The comparability of HbA1c concentrations was assessed by Bland-Altman plot. Results Median (IQR) HbA1c concentration was 45.3 (36.6-61.2) mmol/mol for fresh and 45.3 (36.6-60.6) mmol/mol for frozen/thawed samples. No significant difference in HbA1c concentrations was found comparing fresh and frozen/thawed samples (P = 0.070) in the whole group, as well as in healthy and diabetic subjects. The median calculated bias between fresh and frozen/thawed samples was 0% in whole group and healthy subjects, and 1.19% in diabetic patients. No significant difference was found between the biases according to baseline HbA1c values (P = 0.150). The Bland-Altman plot analysis showed a positive bias of 0.4% (95% CI: - 2.8 - 3.7%), which indicates high compliance between HbA1c values and no relevant influence of sample freezing on clinical significance of HbA1c measurement. Conclusions Storage for up to 12 weeks at - 80 °C with a single freeze/thaw cycle does not affect HbA1c concentrations measured with HPLC method on Bio-Rad D-10® analyzer. |
| وصف الملف: | application/pdf |
| تدمد: | 1846-7482 1330-0962 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac43c5f0efa8f4e9ebfd8eb46e008c39Test https://doi.org/10.11613/bm.2016.038Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ac43c5f0efa8f4e9ebfd8eb46e008c39 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18467482 13300962 |
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