التفاصيل البيبلوغرافية
| العنوان: |
Functional consequence of targeting protein kinase B/Akt to GLUT4 vesicles |
| المؤلفون: |
Ducluzeau, Pierre-Henri, Fletcher, Laura M., Welsh, Gavin I., Tavaré, Jeremy M. |
| بيانات النشر: |
Company of Biologists |
| سنة النشر: |
2002 |
| المجموعة: |
HighWire Press (Stanford University) |
| مصطلحات موضوعية: |
Research Article |
| الوصف: |
We have investigated the role of protein kinase B (Akt) in the insulin-stimulated translocation of vesicles containing the insulin-responsive isoform of glucose transporter (GLUT4) to the plasma membrane of adipocytes. Previous reports have suggested that protein kinase B can bind to intracellular GLUT4 vesicles in an insulin-dependent manner, but the functional consequence of this translocation is not known. In this study we have artificially targeted constitutively active and kinase-inactive mutants of protein kinase B to intracellular GLUT4 vesicles by fusing them with the N-terminus of GLUT4 itself. We examined the effect of these mutants on the insulin-dependent translocation of the insulin-responsive amino peptidase IRAP (a bona fide GLUT4-vesicle-resident protein). A kinase-inactive protein kinase B targeted to GLUT4 vesicles was an extremely effective dominant-negative inhibitor of insulin-stimulated IRAP translocation to the plasma membrane. By contrast, a kinase-inactive protein kinase B expressed in the cytoplasm did not have an effect. The results suggest that protein kinase B has an important functional role at, or in the vicinity of, GLUT4 vesicles in the insulin-dependent translocation of those vesicles to the plasma membrane of adipocytes. |
| نوع الوثيقة: |
text |
| وصف الملف: |
text/html |
| اللغة: |
English |
| العلاقة: |
http://jcs.biologists.org/cgi/content/short/115/14/2857Test |
| الإتاحة: |
http://jcs.biologists.org/cgi/content/short/115/14/2857Test |
| حقوق: |
Copyright (C) 2002, Company of Biologists |
| رقم الانضمام: |
edsbas.9D278367 |
| قاعدة البيانات: |
BASE |
