دورية أكاديمية
Histopathological, immunohistochemical, and molecular study of BHV-5 infection in the central nervous system of experimentally infected calves
| العنوان: | Histopathological, immunohistochemical, and molecular study of BHV-5 infection in the central nervous system of experimentally infected calves |
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| المؤلفون: | Didier Q. Cagnini, Paulo H.J. Cunha, José C.F. Pantoja, Peres R. Badial, José Paes de Oliveira-Filho, João P. Araújo-Junior, Renée Laufer-Amorim, Alexandre S. Borges |
| المصدر: | Pesquisa Veterinária Brasileira, Vol 35, Iss 4, Pp 337-343 (2015) |
| بيانات النشر: | Colégio Brasileiro de Patologia Animal (CBPA) |
| سنة النشر: | 2015 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Herpesvirus bovino 5, doenças de bovinos, meningoencefalite, neuropatologia, Veterinary medicine, SF600-1100 |
| الوصف: | Bovine meningoencephalitis caused by BHV-5, a double-stranded DNA enveloped virus that belongs to the family Herpesviridae and subfamily Alphaherpesvirinae, is an important differential diagnosis of central nervous diseases. The aim of this study was to describe the histological changes in the central nervous system of calves experimentally infected with BHV-5 and compare these changes with the PCR and IHC results. Formalin-fixed paraffin-embedded central nervous system samples from calves previously inoculated with BHV-5 were microscopically evaluated and tested using IHC and PCR. All the animals presented with nonsuppurative meningoencephalitis. From 18 evaluated areas of each calf, 32.41% and 35.19% were positive by IHC and PCR, respectively. The telencephalon presented more accentuated lesions and positive areas in the PCR than other encephalic areas and was the best sampling area for diagnostic purposes. Positive areas in the IHC and PCR were more injured than IHC and PCR negative areas. The animal with neurological signs showed more PCR- and IHC-positive areas than the other animals. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English Portuguese |
| تدمد: | 1678-5150 |
| العلاقة: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000400337&lng=en&tlng=enTest; https://doaj.org/toc/1678-5150Test; https://doaj.org/article/2d096b49e4ad471b834eb87f0e7859cbTest |
| DOI: | 10.1590/S0100-736X2015000400004 |
| الإتاحة: | https://doi.org/10.1590/S0100-736X2015000400004Test https://doaj.org/article/2d096b49e4ad471b834eb87f0e7859cbTest |
| رقم الانضمام: | edsbas.5AA9B29 |
| قاعدة البيانات: | BASE |
| تدمد: | 16785150 |
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| DOI: | 10.1590/S0100-736X2015000400004 |