دورية أكاديمية
Direct quantification of 3' terminal 2'-O-methylation of small RNAs by RT-qPCR
| العنوان: | Direct quantification of 3' terminal 2'-O-methylation of small RNAs by RT-qPCR |
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| المؤلفون: | Wang, Nan, Qu, Shuang, Sun, Wu, Zeng, Ziyi, Liang, Hongwei, Zhang, Chen-Yu, Chen, Xi, Zen, Ke |
| بيانات النشر: | Cold Spring Harbor Laboratory Press |
| سنة النشر: | 2018 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | ARTICLE |
| الوصف: | Modification of nucleotides significantly increases the diversity of functional nucleic acids. As one of the most common modifications of RNAs, methylation of the 2′-hydroxyl-group of ribonucleotides (2′- O -methylation) has been found in various RNAs in eukaryotes. However, due to the lack of an efficient method for quantifying small RNA 3′ terminal 2′- O -methylation, it is difficult to monitor the dynamic change of 3′ terminal 2′- O -methylation during various biological processes. Capitalizing on the finding that 3′ terminal RNA 2′- O -methylation can inhibit the activity of poly(A) polymerase, an enzyme that can add the poly(A)-tail to RNA, we develop a method by which the 2′- O -methylation level of small RNAs, such as microRNAs (miRNAs) and Piwi-interacting RNAs (piRNAs), can be directly quantified based on the poly(A)-tailed RT-qPCR technique. With this method, we successfully determine the 2′- O -methylation level of miRNAs in Arabidopsis thaliana and mouse lung tissue, piRNA in human seminal plasma, and monitor the alteration of miRNA 2′- O -methylation in Drosophila Schneider 2 cells after knockdown of Drosophila methyltransferase protein Hua enhancer 1 (DmHen-1). |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://rnajournal.cshlp.org/cgi/content/short/24/11/1520Test; http://dx.doi.org/10.1261/rna.065144.117Test |
| DOI: | 10.1261/rna.065144.117 |
| الإتاحة: | https://doi.org/10.1261/rna.065144.117Test http://rnajournal.cshlp.org/cgi/content/short/24/11/1520Test |
| حقوق: | Copyright (C) 2018, RNA Society |
| رقم الانضمام: | edsbas.50C223BF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1261/rna.065144.117 |
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