Imaging Mouse Embryonic Cardiovascular Development
| العنوان: | Imaging Mouse Embryonic Cardiovascular Development |
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| المؤلفون: | Mary E. Dickinson, Kirill V. Larin, Tegy J. Vadakkan, Irina V. Larina, Monica D. Garcia |
| المصدر: | Cold Spring Harbor Protocols. 2012:pdb.top071498 |
| بيانات النشر: | Cold Spring Harbor Laboratory, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Microscopy, Confocal, Staining and Labeling, medicine.diagnostic_test, Cell morphogenesis, Dynamic imaging, Biology, Cardiovascular System, Embryonic stem cell, Fluorescence, General Biochemistry, Genetics and Molecular Biology, law.invention, Cell biology, Mice, Optical coherence tomography, Confocal microscopy, law, Live cell imaging, Microscopy, Image Processing, Computer-Assisted, Transgenic lines, medicine, Animals, Tomography, Optical Coherence |
| الوصف: | Early development of the mammalian cardiovascular system is a highly dynamic process. Live imaging is an essential tool for analyzing normal and abnormal cardiovascular development and dynamics. This article describes two optical approaches for live dynamic imaging of mouse embryonic cardiovascular development: confocal microscopy and optical coherence tomography (OCT). Confocal microscopy, used in combination with fluorescent protein reporter lines, enables visualization of the developing and remodeling cardiovascular system with submicron resolution and even allows visualization of subcellular details of labeled structures. We describe mouse transgenic lines that can be used to image the developing vasculature and characterize hemodynamics by tracking individual blood cells. Confocal microscopy of vital fluorescent markers reveals unique details about cell morphogenesis and movement; however, the imaging depth of this method is limited to ∼200 µm. This limitation can be addressed by using OCT, which allows three-dimensional (3D) imaging millimeters into tissue, although this is achieved at the expense of lower spatial resolution (2–10 µm). We describe here how OCT can be applied to the structural analysis of developing mouse embryos and hemodynamic analysis in deep embryonic vessels. These complementary approaches can be used to analyze cardiovascular defects in mutant animals to understand genetic signaling pathways regulating human development. |
| تدمد: | 1559-6095 1940-3402 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9d8e97b0b4dc64a2cfb819115909918Test https://doi.org/10.1101/pdb.top071498Test |
| رقم الانضمام: | edsair.doi.dedup.....c9d8e97b0b4dc64a2cfb819115909918 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15596095 19403402 |
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