Metaproteomics Reveals Potential Mechanisms by which Dietary Resistant Starch Supplementation Attenuates Chronic Kidney Disease Progression in Rats
| العنوان: | Metaproteomics Reveals Potential Mechanisms by which Dietary Resistant Starch Supplementation Attenuates Chronic Kidney Disease Progression in Rats |
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| المؤلفون: | Yasir Rahmatallah, Alan J. Tackett, Oleg Karaduta, Stephanie D. Byrum, Dorothy A. Kieffer, Dmitri S. Andreyev, Taylor McElroy, Roy J. Martin, Sean H. Adams, Nosratola D. Vaziri, Lisa M. Orr, Boris Zybailov, John M. Arthur, Galina V. Glazko, Ricky D. Edmondson, Samuel G. Mackintosh |
| المساهمون: | Nie, Daotai |
| المصدر: | PLoS ONE Zybailov, Boris L; Glazko, Galina V; Rahmatallah, Yasir; Andreyev, Dmitri S; McElroy, Taylor; Karaduta, Oleg; et al.(2019). Metaproteomics reveals potential mechanisms by which dietary resistant starch supplementation attenuates chronic kidney disease progression in rats.. PloS one, 14(1), e0199274. doi: 10.1371/journal.pone.0199274. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/5zp1p5gfTest PLoS ONE, Vol 14, Iss 1, p e0199274 (2019) PloS one, vol 14, iss 1 |
| بيانات النشر: | Cold Spring Harbor Laboratory, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Proteomics, Metabolic Processes, Kidney Disease, Proteome, medicine.medical_treatment, Cell Membranes, 030232 urology & nephrology, Pharmacology, Gut flora, Biochemistry, Starches, Rats, Sprague-Dawley, Binding Analysis, Database and Informatics Methods, Cecum, 0302 clinical medicine, Ruminococcus, Chronic Kidney Disease, Medicine and Health Sciences, Renal Insufficiency, Chronic, Database Searching, Resistant starch, Data Management, 2. Zero hunger, 0303 health sciences, Multidisciplinary, Organic Compounds, Starch, Sprague dawley, Chemistry, Untargeted metabolomics, medicine.anatomical_structure, Nephrology, Physical Sciences, Disease Progression, Medicine, Cellular Structures and Organelles, Cell Binding Assay, Research Article, Ruminococcaceae, Computer and Information Sciences, food.ingredient, General Science & Technology, Science, Carbohydrates, Butyrate, Biology, Research and Analysis Methods, 03 medical and health sciences, food, Bacterial Proteins, Gut bacteria, MD Multidisciplinary, medicine, Animals, Renal Insufficiency, Chronic, Chemical Characterization, Nutrition, Taxonomy, 030304 developmental biology, Prebiotic, Organic Chemistry, Mucin, Chemical Compounds, Biology and Life Sciences, Cell Biology, Intracellular Membranes, medicine.disease, biology.organism_classification, Gastrointestinal Microbiome, Rats, 030104 developmental biology, Metabolism, Metaproteomics, Sprague-Dawley, Kidney disease |
| الوصف: | BackgroundResistant starch is a prebiotic metabolized by the gut bacteria. It has been shown to attenuate chronic kidney disease (CKD) progression in rats. Previous studies employed taxonomic analysis using 16S rRNA sequencing and untargeted metabolomics profiling. Here we expand these studies by metaproteomics, gaining new insight into the host-microbiome interaction.MethodsDifferences between cecum contents in CKD rats fed a diet containing resistant starch with those fed a diet containing digestible starch were examined by comparative metaproteomics analysis. Taxonomic information was obtained using unique protein sequences. Our methodology results in quantitative data covering both host and bacterial proteins.Results5,834 proteins were quantified, with 947 proteins originating from the host organism. Taxonomic information derived from metaproteomics data surpassed previous 16S RNA analysis, and reached species resolutions for moderately abundant taxonomic groups. In particular, the Ruminococcaceae family becomes well resolved – with butyrate producers and amylolytic species such as R. bromii clearly visible and significantly higher while fibrolytic species such as R. flavefaciens are significantly lower with resistant starch feeding. The observed changes in protein patterns are consistent with fiber-associated improvement in CKD phenotype. Several known host CKD-associated proteins and biomarkers of impaired kidney function were significantly reduced with resistant starch supplementation. Data are available via ProteomeXchange with identifier PXD008845.Conclusions- Metaproteomics analysis of cecum contents of CKD rats with and without resistant starch supplementation reveals changes within gut microbiota at unprecedented resolution, providing both functional and taxonomic information. Proteins and organisms differentially abundant with RS supplementation point toward a shift from mucin degraders to butyrate producers. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1101/340513 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::457647ed60a9fe0cca07f11a17ef8639Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....457647ed60a9fe0cca07f11a17ef8639 |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.1101/340513 |
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