رسالة جامعية
'Noncovalent Complexation of Single-Wall Carbon Nanotubes with Biopolymers: Dispersion, Purification, and Protein Interactions'
| العنوان: | 'Noncovalent Complexation of Single-Wall Carbon Nanotubes with Biopolymers: Dispersion, Purification, and Protein Interactions' |
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| المؤلفون: | DiLillo, Ana M. |
| بيانات النشر: | Cleveland State University / OhioLINK, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | Ohiolink ETDs |
| Original Material: | http://rave.ohiolink.edu/etdc/view?acc_num=csu1624461866858216Test |
| مصطلحات موضوعية: | Chemical Engineering, Engineering, Materials Science, Nanoscience, Nanotechnology, Single-Wall Carbon Nanotubes, Purification, Lectin Interactions, Glycopolymers, Noncovalent Functionalization, DNA Deprotonation |
| الوصف: | This thesis is a comprehensive study on the noncovalent complexation of single-wall carbon nanotubes (SWCNTs) using biopolymers, including single-stranded DNA and synthetic glycopolymers to explore their potential applications as optical sensors. SWCNTs are cylindrical structures of carbon lattice with diameters of ≈ 1 nanometer. They have unique electronic, chemical, and optical properties, which make them ideal candidates for bioimaging, biosensing, and drug delivery applications. The resulting biopolymer-SWCNT complexes maintain the intrinsic properties of nanotubes and possess specific biological functionalities as well. Here, we have extensively studied the structure-property relationship of two types of biopolymer-complexed SWCNT systems, namely DNA- and glycopolymer-wrapped SWCNTs (i.e., DNA-SWCNTs and Glyco-SWCNTs). First, we investigated the optical properties of DNA-SWCNTs utilizing (GT)n sequences, where n = 6, 10, 15, 20, at controlled pH to examine the effect of DNA nucleobase deprotonation on the near-infrared (NIR) fluorescence of SWCNTs and the subsequent SWCNT separation. When increasing the pH of aqueous dispersions of DNA-SWCNTs to a basic region (i.e., pH ≈ 12) the NIR emission intensity of nanotubes increased indicating the conformational change of DNA on the surface of nanotubes. The resulting DNA-SWCNT complexes distributed unevenly in a polymer aqueous two-phase system, allowing SWCNT separation at controlled pH. Next, we studied Glyco-SWCNT complexes and their ability to interact with carbohydrate-binding proteins (i.e., lectins). We utilized a disaccharide lactose-containing homopolymer with a polymer chain length of n = 400 (i.e., Lact-AM 400) to investigate the dispersion quality and carbohydrate-protein binding interactions of Glyco-SWCNTs. Various lectins were utilized including glucose and mannose-binding Concanavalin A(ConA) and galactose-binding Arachis hypogaea (PNA) to determine carbohydrate-protein interactions which were compared to mannose-binding Galanthus nivalis (GNA) and Bovine serum albumin (BSA) that does not bind specifically to carbohydrates. ConA showed a greater ability to cause the cross linking and aggregations of Glyco-SWCNTswhile PNA reacted faster with Glyco-SWCNTs based on the kinetics of protein interactions. Taken together, our findings provide insights for creating biopolymer-SWCNT complexes with controlled optical properties and biological functionalities. This opens new possibilities for designing novel nanosystems for many applications, from nanotube purification to biological sensing and imaging to nanomedicine. |
| Original Identifier: | oai:etd.ohiolink.edu:csu1624461866858216 |
| نوع الوثيقة: | text |
| اللغة: | English |
| الإتاحة: | http://rave.ohiolink.edu/etdc/view?acc_num=csu1624461866858216Test |
| حقوق: | unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| رقم الانضمام: | edsndl.OhioLink.oai.etd.ohiolink.edu.csu1624461866858216 |
| قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
| الوصف غير متاح. |