دورية أكاديمية
Effect of human epididymis protein 4 gene silencing on the malignant phenotype in ovarian cancer
| العنوان: | Effect of human epididymis protein 4 gene silencing on the malignant phenotype in ovarian cancer |
|---|---|
| المؤلفون: | Zou Shu-li, Chang Xiao-hong, Ye Xue, Cheng Hong-yan, Cheng Ye-xia, Tang Zhi-jian, Zhang Zu-juan, Gao Li, Chen Xin-hua, Cui Heng |
| المساهمون: | Cui, H (reprint author), Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China., Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China. |
| المصدر: | PubMed ; SCI |
| بيانات النشر: | Chinese Medical Journal |
| سنة النشر: | 2011 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | human epididymis protein 4, RNA interference, ovarian carcinoma, carcinogenesis, BREAST-CANCER, EXPRESSION PROFILES, EPITHELIAL-CELLS, CDNA MICROARRAY, CARCINOMAS, HE-4, HYBRIDIZATION, INHIBITOR, MARKERS, DIFFERENTIATION |
| الوصف: | Background Human epididymis secretory protein 4 (HE4) has been proved to be a promising novel biomarker for the detection of epithelial ovarian carcinomas. Compared with CA125, HE4 assay demonstrated an improved ability to discriminate between pelvic mass with malignant and benign disease. Though it is well known that HE4 is overexpressed in ovarian cancer, however, the role of HE4 in the carcinogenesis and progression of ovarian cancer remains unkown. Methods In this study, we explored the role of HE4 in the carcinogenesis and progression of ovarian cancer. We screened nine ovarian cancer cell lines for HE4 expression, and using RNA interference (RNAi), we silenced HE4 gene expression in CaoV3 and SKOV3.ip1 ovarian cancer cell lines. We assessed the effect of HE4 gene silencing on the transformed phenotype by examining the cell cycle, apoptosis, proliferation and transwell migration/invasion in vitro. Results HE4 gene silencing induces G0/G1 arrest and blocks the progression from the G1 to S phase in CaoV3 and SKOV3.ip1 cells. HE4 knockdown also inhibited cell proliferation, migration and invasion in SKOV3.ip1 cells in vitro. Conclusion HE4 may be involved in the regulation of the cell cycle and promote ovarian cancer migration and invasion. Chin Med J 2011;124(19):3133-3140 ; Medicine, General & Internal ; SCI(E) ; PubMed ; 中国科技核心期刊(ISTIC) ; 中国科学引文数据库(CSCD) ; 5 ; ARTICLE ; 19 ; 3133-3140 ; 124 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 0366-6999 |
| العلاقة: | CHINESE MEDICAL JOURNAL.2011,124,(19),3133-3140.; 662200; http://hdl.handle.net/20.500.11897/307303Test; WOS:000296307600033 |
| DOI: | 10.3760/cma.j.issn.0366-6999.2011.19.032 |
| الإتاحة: | https://doi.org/20.500.11897/307303Test https://doi.org/10.3760/cma.j.issn.0366-6999.2011.19.032Test https://hdl.handle.net/20.500.11897/307303Test |
| رقم الانضمام: | edsbas.F7ECA709 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 03666999 |
|---|---|
| DOI: | 10.3760/cma.j.issn.0366-6999.2011.19.032 |