Synergistic antitumor efficacy of oncolytic adenovirus combined with chemotherapy
| العنوان: | Synergistic antitumor efficacy of oncolytic adenovirus combined with chemotherapy |
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| المؤلفون: | Chuan-hua Zhao, Zhi-qiang Li, Changqing Su, Qi Zhang, Zefei Jiang, Fei-jiao Ge, Yi-mei Qu, Yue-min Li, Santai Song, Qi-jun Qian |
| المصدر: | Chinese Journal of Cancer Research. 19:76-81 |
| بيانات النشر: | Chinese Journal of Cancer Research, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Oncolytic adenovirus, Cancer Research, Adenovirus genome, Biology, medicine.disease, Cell killing, Breast cancer, Oncology, Cancer cell, Cancer research, medicine, Telomerase reverse transcriptase, Virotherapy, Cytotoxicity |
| الوصف: | Chemotherapy is an effective means of treating breast cancer, and cancer-specific replicative adenovirus is also a promising antitumor agent in recent years. Our investigation aims to demonstrate that CNHK300 can mediate selective antitumor efficacy and produce synergistic cytotoxicity with chemotherapy on HER-2 over-expressing breast cancer. We engineered the telomerase-dependent replicative adenovirus CNHK300 by placing the E1A gene under the control of the human hTERT promoter. By analysis of E1A expression, we proved the fidelity of hTERT promoter in adenovirus genome and the selective expression of E1A in telomerase-positive breast cancer cells but not in normal fibroblast cells. By proliferation test, we further showed efficient replication of CNHK300 in breast cancer cells with apparently attenuated proliferation in normal fibroblast cells. Finally, we demonstrated by MTT methods that CNHK300 virus caused potent cytolysis and produced synergistic cytotoxicity with chemotherapy in breast cancer cells with attenuated cytotoxicity on normal cells. In this virus, the E1A gene is successfully placed under the control of the human hTERT promoter. CNHK300 virus replicated as efficiently as the wild-type adenovirus and caused intensive cell killing in HER-2 over-expressing breast cancer cells in vitro. In contrast, telomerase-negative normal fibroblast cells, which expressed no hTERT activity, were not able to support CNHK300 replication. Combined treatment of CNHK300 with paclitaxel improved cytotoxicity on cancer cells. We conclude that CNHK300 can produce selective antitumor efficacy and enhance the in vitro response of chemotherapy on HER-2 overexpressing breast cancer. |
| تدمد: | 1993-0631 1000-9604 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::549c15703ebba8a3bbb5e88868b07e1fTest https://doi.org/10.1007/s11670-007-0076-7Test |
| رقم الانضمام: | edsair.doi...........549c15703ebba8a3bbb5e88868b07e1f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19930631 10009604 |
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