دورية أكاديمية
Comparative Analysis of H&E and Prussian Blue Staining in a Mouse Model of Cerebral Microbleeds
| العنوان: | Comparative Analysis of H&E and Prussian Blue Staining in a Mouse Model of Cerebral Microbleeds |
|---|---|
| المؤلفون: | Liu, Shuo, Grigoryan, Mher Mahoney, Vasilevko, Vitaly, Sumbria, Rachita K., Paganini-Hill, Annlia, Cribbs, David H., Fisher, Mark J. |
| المصدر: | Pharmacy Faculty Articles and Research |
| بيانات النشر: | Chapman University Digital Commons |
| سنة النشر: | 2014 |
| المجموعة: | Chapman University Digital Commons |
| مصطلحات موضوعية: | Cerebral microbleeds, H&E, Prussian Blue, LPS, Animal Experimentation and Research, Medical Neurobiology, Nervous System Diseases, Other Chemicals and Drugs, Other Pharmacy and Pharmaceutical Sciences, Pharmaceutical Preparations, Therapeutics |
| الوصف: | Cerebral microbleeds are microscopic hemorrhages with deposits of blood products in the brain, which can be visualized with MRI and are implicated in cerebrovascular diseases. Hematoxylin and eosin (H&E) and Perl’s Prussian blue are popular staining methods used to localize cerebral microbleeds in pathology. This paper compared these two staining techniques in a mouse model of cerebral microbleeds. We used lipopolysaccharide (LPS) to induce cerebral microhemorrhages. C57B6 mice were treated with LPS (5 mg/kg, i.p.) or vehicle at baseline and at 24 hr. The brains were extracted 48 hr after the first injection and adjacent coronal sections were stained with H&E and Prussian blue to compare the effectiveness of the two staining techniques. H&E-positive stains were increased with LPS treatment and were correlated with grossly visible microhemorrhages on the brain surface; Prussian blue-positive stains, by comparison, showed no significant increase with LPS treatment and did not correlate with either H&E-positive stains or surface microhemorrhages. H&E staining is thus a more reliable indicator of acute bleeding events induced by LPS in this model within a short time span. |
| نوع الوثيقة: | text |
| اللغة: | unknown |
| العلاقة: | https://digitalcommons.chapman.edu/pharmacy_articles/866Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6728446Test/ |
| الإتاحة: | https://digitalcommons.chapman.edu/pharmacy_articles/866Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6728446Test/ |
| حقوق: | The authors |
| رقم الانضمام: | edsbas.8C3113D4 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |