The effect of hypoxia on intermediary metabolism and oxidative status in gilthead sea bream (Sparus aurata) fed on diets supplemented with methionine and white tea
| العنوان: | The effect of hypoxia on intermediary metabolism and oxidative status in gilthead sea bream (Sparus aurata) fed on diets supplemented with methionine and white tea |
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| المؤلفون: | Amalia Pérez-Jiménez, Helena Peres, Aires Oliva-Teles, V.C. Rubio |
| المصدر: | e-IEO. Repositorio Institucional Digital de Acceso Abierto del Instituto Español de Oceanografía instname |
| بيانات النشر: | Centro Oceanográfico de Murcia, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Blood Glucose, medicine.medical_specialty, Antioxidant, Time Factors, Acuicultura, Physiology, Health, Toxicology and Mutagenesis, medicine.medical_treatment, Glutathione reductase, Biology, Glucosephosphate Dehydrogenase, Toxicology, medicine.disease_cause, Biochemistry, Antioxidants, Superoxide dismutase, chemistry.chemical_compound, Methionine, Internal medicine, medicine, Animals, Centro Oceanográfico de Murcia, Hypoxia, Enzyme Assays, Glutathione Peroxidase, L-Lactate Dehydrogenase, Tea, Superoxide Dismutase, Cell Biology, General Medicine, Glutathione, Hypoxia (medical), Catalase, Sea Bream, Diet, Enzyme Activation, Isoenzymes, Oxidative Stress, Endocrinology, chemistry, Liver, Dietary Supplements, biology.protein, medicine.symptom, Oxidation-Reduction, Oxidative stress |
| الوصف: | The present study evaluates the influence of previous nutritional status, fish fed on diets supplemented with tea and methionine, on acute hypoxia tolerance and subsequent recovery of Sparus aurata juveniles. Four isonitrogenous (45% of protein) and isolipidic (18% lipid) diets were formulated to contain 0.3% methionine, 2.9% white tea dry leaves or 2.9% of white tea dry leaves+0.3% methionine. An unsupplemented diet was used as control. Hepatic key enzymes of intermediary metabolism and antioxidant status, superoxide dismutase isoenzyme profile, glutathione (total, reduced and oxidized) and oxidative damage markers were determined under normoxia, hypoxia challenge and during normoxia recovery. Dietary white tea inclusion decreased plasma glucose levels under normoxia and seemed to induce an increase in anaerobic pathways as showed by enhanced liver lactate dehydrogenase activity. Hypoxia challenge reversed some of the responses induced by diet tea supplementation. Hypoxia decreased plasma glucose levels, increased glucose 6-P-dehydrogeanse activity, decreased superoxide dismutase activity (especially Mn-SOD and CuZn-SOD isoforms) and increased glutathione peroxidase activity in all dietary treatments. Catalase activity during hypoxia varied with dietary treatments and glutathione reductase was not modified. Antioxidant defenses were insufficient to avoid an oxidative stress condition under hypoxia, independently of dietary treatment. In general, pre-challenge values were recovered for almost all parameters within 6 h recovery time. |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7635829556128594621368b03aacbc89Test https://hdl.handle.net/10508/8390Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7635829556128594621368b03aacbc89 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |