Fibrin biopolymer as scaffold candidate to treat bone defects in rats
| العنوان: | Fibrin biopolymer as scaffold candidate to treat bone defects in rats |
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| المؤلفون: | Rui Seabra Ferreira, Luis A. Justulin, Marjorie de Assis Golim, Danuta Pulz Doiche, Patrícia Rodrigues de Lima, Alexandre Leite Rodrigues de Oliveira, Mateus Vidigal de Castro, Elenize Jamas Pereira, Claudia Vilalva Cassaro, Natalia P. Biscola, Benedito Barraviera |
| المساهمون: | Universidade Estadual Paulista (Unesp), Universidade Estadual de Campinas (UNICAMP) |
| المصدر: | Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 25, Article number: e20190027, Published: 04 NOV 2019 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 25 (2019) The Journal of Venomous Animals and Toxins Including Tropical Diseases Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Journal of Venomous Animals and Toxins including Tropical Diseases v.25 2019 The Journal of venomous animals and toxins including tropical diseases |
| بيانات النشر: | Centro de Estudos de Venenos e Animais Peçonhentos, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Scaffold, Fibrin sealant, RC955-962, Phosphate, Biphasic calcium, Bone healing, Toxicology, Bone tissue, Fibrin, Biphasic calcium phosphate, Biomaterials, Tissue engineering, Arctic medicine. Tropical medicine, RA1190-1270, medicine, Bone regeneration, biology, Chemistry, Fibrin biopolymer, Research, Mesenchymal stem cell, Infectious Diseases, medicine.anatomical_structure, QL1-991, Toxicology. Poisons, biology.protein, Mesenchymal stem cells, Animal Science and Zoology, Parasitology, Stem cell, Zoology, Biomedical engineering |
| الوصف: | Made available in DSpace on 2020-12-12T01:47:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01. Added 1 bitstream(s) on 2021-07-15T15:25:38Z : No. of bitstreams: 1 S1678-91992019000100320.pdf: 9344735 bytes, checksum: 7b646d4f4d4f8d668504f23b146aca32 (MD5) Background: Bone tissue repair remains a challenge in tissue engineering. Currently, new materials are being applied and often integrated with live cells and biological scaffolds. The fibrin biopolymer (FBP) proposed in this study has hemostatic, sealant, adhesive, scaffolding and drug-delivery properties. The regenerative potential of an association of FBP, biphasic calcium phosphate (BCP) and mesenchymal stem cells (MSCs) was evaluated in defects of rat femurs. Methods: Adult male Wistar rats were submitted to a 5-mm defect in the femur. This was filled with the following materials and/or associations: BPC; FBP and BCP; FBP and MSCs; and BCP, FBP and MSCs. Bone defect without filling was defined as the control group. Thirty and sixty days after the procedure, animals were euthanatized and subjected to computed tomography, scanning electron microscopy and qualitative and quantitative histological analysis. Results: It was shown that FBP is a suitable scaffold for bone defects due to the formation of a stable clot that facilitates the handling and optimizes the surgical procedures, allowing also cell adhesion and proliferation. The association between the materials was biocompatible. Progressive deposition of bone matrix was higher in the group treated with FBP and MSCs. Differentiation of mesenchymal stem cells into osteogenic lineage was not necessary to stimulate bone formation. Conclusions: FBP proved to be an excellent scaffold candidate for bone repair therapies due to application ease and biocompatibility with synthetic calcium-based materials. The satisfactory results obtained by the association of FBP with MSCs may provide a more effective and less costly new approach for bone tissue engineering. Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP) Botucatu Medical School (FMB) São Paulo State University (UNESP) Extracellular Matrix Laboratory Botucatu Biosciences Institute (IBB) São Paulo State University (UNESP) Flow Cytometry Laboratory Blood Center Botucatu Medical School (FMB) São Paulo State University (UNESP) Department of Structural and Functional Biology Biosciences Institute (IB) University of Campinas (UNICAMP) Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Husbandry São Paulo State University (UNESP) Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP) Botucatu Medical School (FMB) São Paulo State University (UNESP) Extracellular Matrix Laboratory Botucatu Biosciences Institute (IBB) São Paulo State University (UNESP) Flow Cytometry Laboratory Blood Center Botucatu Medical School (FMB) São Paulo State University (UNESP) Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Husbandry São Paulo State University (UNESP) |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 1678-9199 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bd3f0f41e5bc305098663b1be26a0f2Test http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100320&lng=en&tlng=enTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8bd3f0f41e5bc305098663b1be26a0f2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16789199 |
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