Dose-Dependent Effect of Dietary Conjugated Linoleic Acid on the Growth of Rat Hepatoma dRLh-84 Cells In Vivo
| العنوان: | Dose-Dependent Effect of Dietary Conjugated Linoleic Acid on the Growth of Rat Hepatoma dRLh-84 Cells In Vivo |
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| المؤلفون: | Akira Hirao, Keizo Sugimachi, Koji Yamada, Tatsuya Rikimaru, Yoko Tanaka, Masayo Yamasaki, Mitsuo Shimada, Atsushi Ikeda, Hirofumi Tachibana |
| المصدر: | Journal of Nutritional Science and Vitaminology. 48:505-511 |
| بيانات النشر: | Center for Academic Publications Japan, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Conjugated linoleic acid, Linoleic acid, Medicine (miscellaneous), Prostaglandin, Biology, Dinoprostone, Linoleic Acid, chemistry.chemical_compound, Liver Neoplasms, Experimental, Dietary Fats, Unsaturated, In vivo, Internal medicine, medicine, Animals, RNA, Messenger, Phospholipids, Nutrition and Dietetics, Dose-Response Relationship, Drug, integumentary system, Reverse Transcriptase Polymerase Chain Reaction, Body Weight, food and beverages, Organ Size, Rats, Isoenzymes, Transplantation, Blotting, Southern, Dose–response relationship, Endocrinology, Liver, chemistry, Cyclooxygenase 2, Prostaglandin-Endoperoxide Synthases, biology.protein, lipids (amino acids, peptides, and proteins), Arachidonic acid, Cyclooxygenase, Cell Division |
| الوصف: | In this study, the effect of varying doses of conjugated linoleic acid (CLA) on the growth of transplanted hepatoma dRLh-84 cells and the relationship between tumor growth and prostaglandin (PG) E2 production or cyclooxygenase (COX)-2 expression were examined. Donryu rats were fed an experimental diet containing 0, 0.1, 0.5, or 2 wt.% CLA for 3 wk, and then dRLh-84 cells were transplanted into the liver. Results show that dietary CLA (0.5 and 2 wt.%) significantly enhanced the growth of the transplanted hepatoma cells compared to the non-CLA diet group at 20 d after cell transplantation. Tumor weight at 10 d after transplantation was also significantly higher in the 2 wt.% CLA group than in non-CLA fed rats. Ten days after transplantation, the PGE2 level in the tumor tissue was shown to be depressed in a CLA dose-dependent manner. Cyclooxygenase-2 (COX-2) mRNA expression in the tumor also tended to be lower in the CLA group than in the non-CLA diet group 10 d after transplantation. Dietary CLA did not affect the tumor phospholipid arachidonic acid level, which is a substrate for PG synthesis. These results indicate that dietary CLA of at least 0.5 wt.% enhances the growth of transplanted dRLh-84 cells in vivo. It is believed that growth promotion of dRLh-84 cells in vivo by CLA cannot be clarified by the PG synthesis dependent mechanism. |
| تدمد: | 1881-7742 0301-4800 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6f542f1c6ef2b205d3bd4284bacb0caTest https://doi.org/10.3177/jnsv.48.505Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c6f542f1c6ef2b205d3bd4284bacb0ca |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18817742 03014800 |
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