دورية أكاديمية
Membrane Tension Gates ERK-Mediated Regulation of Pluripotent Cell Fate.
| العنوان: | Membrane Tension Gates ERK-Mediated Regulation of Pluripotent Cell Fate. |
|---|---|
| المؤلفون: | De Belly, Henry, Stubb, Aki, Yanagida, Ayaka, Labouesse, Céline, Jones, Philip H, Paluch, Ewa K, Chalut, Kevin J |
| بيانات النشر: | Elsevier BV //dx.doi.org/10.1016/j.stem.2020.10.018 Cell Stem Cell |
| سنة النشر: | 2021 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | Beta-catenin, Cell fate choice, Cell surface mechanics, ERK, Embryonic stem cells, Endocytosis, Membrane tension, mechanical signalling, pluripotency, Animals, Cell Differentiation, Mice, Mouse Embryonic Stem Cells, Signal Transduction |
| الوصف: | Cell fate transitions are frequently accompanied by changes in cell shape and mechanics. However, how cellular mechanics affects the instructive signaling pathways controlling cell fate is poorly understood. To probe the interplay between shape, mechanics, and fate, we use mouse embryonic stem cells (ESCs), which change shape as they undergo early differentiation. We find that shape change is regulated by a β-catenin-mediated decrease in RhoA activity and subsequent decrease in the plasma membrane tension. Strikingly, preventing a decrease in membrane tension results in early differentiation defects in ESCs and gastruloids. Decreased membrane tension facilitates the endocytosis of FGF signaling components, which activate ERK signaling and direct the exit from the ESC state. Increasing Rab5a-facilitated endocytosis rescues defective early differentiation. Thus, we show that a mechanically triggered increase in endocytosis regulates early differentiation. Our findings are of fundamental importance for understanding how cell mechanics regulates biochemical signaling and therefore cell fate. ; This work was supported b\ the European Union¶s Hori]on 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 641639 (ITN Biopol, HdB and EKP), the Medical Research Council UK (MRC programme award MC_UU_12018/5, HdB and EKP), the Human Frontier Science Program (Young InvestigatorGrant RGY 66/2013 to EKP), the Leverhulme Trust (Prize in Biological Sciences to EKP), an ERC Consolidator Grant (CellFateTech, 772798, KC), a core support grant from the Wellcome Trust and Medical Research Council to the Wellcome Trust - Medical Research Council Cambridge Stem Cell Institute (KJC). KJC is a Royal Society University Research Fellow. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | Print-Electronic; application/pdf |
| اللغة: | English |
| العلاقة: | https://www.repository.cam.ac.uk/handle/1810/312672Test |
| DOI: | 10.17863/CAM.59770 |
| الإتاحة: | https://doi.org/10.17863/CAM.59770Test https://www.repository.cam.ac.uk/handle/1810/312672Test |
| حقوق: | Attribution-NonCommercial-NoDerivatives 4.0 International ; https://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: | edsbas.E992D30B |
| قاعدة البيانات: | BASE |
| DOI: | 10.17863/CAM.59770 |
|---|