دورية أكاديمية
Long-Range Enhancer Interactions Are Prevalent in Mouse Embryonic Stem Cells and Are Reorganized upon Pluripotent State Transition.
| العنوان: | Long-Range Enhancer Interactions Are Prevalent in Mouse Embryonic Stem Cells and Are Reorganized upon Pluripotent State Transition. |
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| المؤلفون: | Novo, Clara Lopes, Javierre, Biola-Maria, Cairns, Jonathan, Segonds-Pichon, Anne, Wingett, Steven W, Freire-Pritchett, Paula, Furlan-Magaril, Mayra, Schoenfelder, Stefan, Fraser, Peter, Rugg-Gunn, Peter J |
| بيانات النشر: | Elsevier BV //dx.doi.org/10.1016/j.celrep.2018.02.040 Cell Rep |
| سنة النشر: | 2018 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | chromatin looping, differentiation, epigenetics, gene regulation, genome organization, pluripotency, promoter capture Hi-C, Animals, Cell Differentiation, Enhancer Elements, Genetic, Gene Regulatory Networks, Germ Layers, Mice, Mouse Embryonic Stem Cells, Nanog Homeobox Protein, Pluripotent Stem Cells, Promoter Regions, Protein Binding |
| الوصف: | Transcriptional enhancers, including super-enhancers (SEs), form physical interactions with promoters to regulate cell-type-specific gene expression. SEs are characterized by high transcription factor occupancy and large domains of active chromatin, and they are commonly assigned to target promoters using computational predictions. How promoter-SE interactions change upon cell state transitions, and whether transcription factors maintain SE interactions, have not been reported. Here, we used promoter-capture Hi-C to identify promoters that interact with SEs in mouse embryonic stem cells (ESCs). We found that SEs form complex, spatial networks in which individual SEs contact multiple promoters, and a rewiring of promoter-SE interactions occurs between pluripotent states. We also show that long-range promoter-SE interactions are more prevalent in ESCs than in epiblast stem cells (EpiSCs) or Nanog-deficient ESCs. We conclude that SEs form cell-type-specific interaction networks that are partly dependent on core transcription factors, thereby providing insights into the gene regulatory organization of pluripotent cells. ; P.J.R.-G. is supported by the Wellcome Trust (WT093736), Biotechnology and Biological Sciences Research Council (BB/M022285/1 and BB/P013406/1), and the European Commission Network of Excellence EpiGeneSys (HEALTH-F4-2010-257082). This work was also supported by the following grants to P.F.: Medical Research Council (MR/L007150/1, MC_UP_1302/1, MC_UP_1302/3, MC_UP_1302/5), and Biotechnology and Biological Sciences Research Council (BB/J004480/1). |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | Print; application/pdf |
| اللغة: | English |
| العلاقة: | https://www.repository.cam.ac.uk/handle/1810/275704Test |
| DOI: | 10.17863/CAM.22969 |
| الإتاحة: | https://doi.org/10.17863/CAM.22969Test https://www.repository.cam.ac.uk/handle/1810/275704Test |
| حقوق: | Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.97B99472 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17863/CAM.22969 |
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