دورية أكاديمية
Liquid-Liquid Phase Separation of Histone Proteins in Cells: Role in Chromatin Organization
| العنوان: | Liquid-Liquid Phase Separation of Histone Proteins in Cells: Role in Chromatin Organization |
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| المؤلفون: | Shakya, Anisha, Park, Seonyoung, Rana, Neha, King, John T. |
| بيانات النشر: | CELL PRESS |
| سنة النشر: | 2020 |
| المجموعة: | ScholarWorks@UNIST (Ulsan National Institute of Science and Technology) |
| الوصف: | Liquid-liquid phase separation (LLPS) of proteins and nucleic acids has emerged as an important phenomenon in membraneless intracellular organization. We demonstrate that the linker histone H1 condenses into liquid-like droplets in the nuclei of HeLa cells. The droplets, observed during the interphase of the cell cycle, are colocalized with DNA-dense regions indicative of heterochromatin. In vitro, H1 readily undergoes LLPS with both DNA and nucleosomes of varying lengths but does not phase separate in the absence of DNA. The nucleosome core particle maintains its structural integrity inside the droplets, as demonstrated by FRET. Unexpectedly, H2A also forms droplets in the presence of DNA and nucleosomes in vitro, whereas the other core histones precipitate. The phase diagram of H1 with nucleosomes is invariant to the nucleosome length at physiological salt concentration, indicating that H1 is capable of partitioning large segments of DNA into liquid-like droplets. Of the proteins tested (H1, core histones, and the heterochromatin protein HP1 alpha), this property is unique to H1. In addition, free nucleotides promote droplet formation of H1 nucleosome in a nucleotide-dependent manner, with droplet formation being most favorable with ATP. Although LLPS of HP1 alpha is known to contribute to the organization of heterochromatin, our results indicate that H1 also plays a role. Based on our study, we propose that H1 and DNA act as scaffolds for phase-separated heterochromatin domains. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0006-3495 |
| العلاقة: | BIOPHYSICAL JOURNAL, v.118, no.3, pp.753 - 764; https://scholarworks.unist.ac.kr/handle/201301/31314Test; 34952; 000511291400022; https://www.sciencedirect.com/science/article/pii/S0006349519344078?via%3DihubTest |
| DOI: | 10.1016/j.bpj.2019.12.022 |
| الإتاحة: | https://doi.org/10.1016/j.bpj.2019.12.022Test https://scholarworks.unist.ac.kr/handle/201301/31314Test https://www.sciencedirect.com/science/article/pii/S0006349519344078?via%3DihubTest |
| رقم الانضمام: | edsbas.9E81A9B1 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00063495 |
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| DOI: | 10.1016/j.bpj.2019.12.022 |