التفاصيل البيبلوغرافية
| العنوان: |
GIT/PIX Condensates Are Modular and Ideal for Distinct Compartmentalized Cell Signaling. |
| المؤلفون: |
Zhu, Jinwei1,2 (AUTHOR) jinwei.zhu@sjtu.edu.cn, Zhou, Qingqing1,3 (AUTHOR), Xia, Yitian1 (AUTHOR), Lin, Lin1,2 (AUTHOR), Li, Jianchao3 (AUTHOR), Peng, Mengjuan1,4 (AUTHOR), Zhang, Rongguang1,4 (AUTHOR) rgzhang@sibcb.ac.cn, Zhang, Mingjie1,3,5 (AUTHOR) mzhang@ust.hk |
| المصدر: |
Molecular Cell. Sep2020, Vol. 79 Issue 5, p782-782. 1p. |
| مصطلحات موضوعية: |
*MULTIENZYME complexes, *FOCAL adhesions, *GTPASE-activating protein, *PHASE separation, *GUANINE nucleotide exchange factors, *OPTICAL lattices |
| مستخلص: |
Enzymes or enzyme complexes can be concentrated in different cellular loci to modulate distinct functional processes in response to specific signals. How cells condense and compartmentalize enzyme complexes for spatiotemporally distinct cellular events is not well understood. Here we discover that specific and tight association of GIT1 and β-Pix, a pair of GTPase regulatory enzymes, leads to phase separation of the complex without additional scaffolding molecules. GIT1/β-Pix condensates are modular in nature and can be positioned at distinct cellular compartments, such as neuronal synapses, focal adhesions, and cell-cell junctions, by upstream adaptors. Guided by the structure of the GIT/PIX complex, we specifically probed the role of phase separation of the enzyme complex in cell migration and synapse formation. Our study suggests that formation of modular enzyme complex condensates via phase separation can dynamically concentrate limited quantities of enzymes to distinct cellular compartments for specific and optimal signaling. • Small GTPase regulatory enzymes GIT and PIX bind to each other very tightly • The GIT/PIX complex forms highly concentrated condensates via phase separation • Formation of GIT/PIX condensates does not require other scaffold molecules • GIT/PIX condensates are modular for signaling in distinct cellular compartments Cells often concentrate limited amounts of enzymes at specific subcellular regions for distinct functions. Zhu et al. show that the small GTPase regulatory enzymes GIT and PIX autonomously form phase-separated condensates without the need for scaffolding molecules. GIT/PIX condensates are modular and positioned at specific cellular compartments for distinct signaling. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
Full Text Finder