التفاصيل البيبلوغرافية
| العنوان: |
Interleukin-33 Deficiency Exacerbates Bone Loss Associated with Porphyromonas Gingivalis-Induced Experimental Periodontitis in Female Mice. |
| المؤلفون: |
Louisy, Agathe, Geoffroy, Valérie, Halgand, Boris, Lapérine, Olivier, Veziers, Joëlle, Caillon, Jocelyne, Guicheux, Jérôme, Lesclous, Philippe, Cloitre, Alexandra |
| المصدر: |
Cellular Physiology & Biochemistry (Cell Physiol Biochem Press GmbH & Co. KG); 2022, Vol. 56 Issue 3, p270-281, 12p |
| مصطلحات موضوعية: |
BONE marrow cells, INTERLEUKIN-33, FRACTURE healing, BONE resorption, PERIODONTITIS, HOMEOSTASIS, LUMBAR vertebrae |
| مستخلص: |
Background/Aims: Interleukin 33 (IL-33) plays a significant role in immunity but its role in bone physiology and periodontitis needs to be further investigated. The aim of this study was to decipher the contribution of IL-33 to bone homeostasis under physiological conditions, and to alveolar bone loss associated with experimental periodontitis (EP) in IL-33 knockout (KO) mice and their wildtype (WT) littermates. Methods: The bone phenotype of IL-33 KO mice was studied in the maxilla, femur, and fifth lumbar vertebra by micro-computed tomography (micro-CT). EP was induced by a ligature soaked with the periopathogen Porphyromonas gingivalis (Pg) around a maxillary molar. Alveolar bone loss was quantified by micro-CT. The resorption parameters were assessed via toluidine blue staining on maxillary sections. In vitro osteoclastic differentiation assays using bone marrow cells were performed with or without lipopolysaccharide from Pg (LPS-Pg). Results: First, we showed that under physiological conditions, IL-33 deficiency increased the trabecular bone volume/total volume ratio (BV/TV) of the maxillary bone in male and female mice, but not in the femur and fifth lumbar vertebra, suggesting an osteoprotective role for IL-33 in a site-dependent manner. The severity of EP induced by Pg-soaked ligature was increased in IL-33 KO mice but in female mice only, through an increase in the number of osteoclasts. Moreover, osteoclastic differentiation from bone marrow osteoclast progenitors in IL-33-deficient female mice is enhanced in the presence of LPS-Pg. Conclusion: Taken together, our data demonstrate that IL-33 plays a sex-dependent osteoprotective role both under physiological conditions and in EP with Pg. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
