دورية أكاديمية

Signal Transduction in Primary Human T Lymphocytes in Altered Gravity During Parabolic Flight and Clinostat Experiments

التفاصيل البيبلوغرافية
العنوان: Signal Transduction in Primary Human T Lymphocytes in Altered Gravity During Parabolic Flight and Clinostat Experiments
المؤلفون: Svantje Tauber, Swantje Hauschild, Katrin Paulsen, Annett Gutewort, Christiane Raig, Eva Hürlimann, Josefine Biskup, Claudia Philpot, Hartwin Lier, Frank Engelmann, Antonella Pantaleo, Augusto Cogoli, Proto Pippia, Liliana E. Layer, Cora S. Thiel, Oliver Ullrich
المصدر: Cellular Physiology and Biochemistry, Vol 35, Iss 3, Pp 1034-1051 (2015)
بيانات النشر: Cell Physiol Biochem Press GmbH & Co KG, 2015.
سنة النشر: 2015
المجموعة: LCC:Physiology
LCC:Biochemistry
مصطلحات موضوعية: T cell activation, Clinorotation, Gravi-sensitivity, Microgravity, Hypergravity, Parabolic flight, Physiology, QP1-981, Biochemistry, QD415-436
الوصف: Background/Aims: Several limiting factors for human health and performance in microgravity have been clearly identified arising from the immune system, and substantial research activities are required in order to provide the basic information for appropriate integrated risk management. The gravity-sensitive nature of cells of the immune system renders them an ideal biological model in search for general gravity-sensitive mechanisms and to understand how the architecture and function of human cells is related to the gravitational force and therefore adapted to life on Earth. Methods: We investigated the influence of altered gravity in parabolic flight and 2D clinostat experiments on key proteins of activation and signaling in primary T lymphocytes. We quantified components of the signaling cascade 1.) in non-activated T lymphocytes to assess the “basal status” of the cascade and 2.) in the process of activation to assess the signal transduction. Results: We found a rapid decrease of CD3 and IL-2R surface expression and reduced p-LAT after 20 seconds of altered gravity in non-activated primary T lymphocytes during parabolic flight. Furthermore, we observed decreased CD3 surface expression, reduced ZAP-70 abundance and increased histone H3-acetylation in activated T lymphocytes after 5 minutes of clinorotation and a transient downregulation of CD3 and stable downregulation of IL-2R during 60 minutes of clinorotation. Conclusion: CD3 and IL-2R are downregulated in primary T lymphocytes in altered gravity. We assume that a gravity condition around 1g is required for the expression of key surface receptors and appropriate regulation of signal molecules in T lymphocytes.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1015-8987
1421-9778
العلاقة: http://www.karger.com/Article/FullText/373930Test; https://doaj.org/toc/1015-8987Test; https://doaj.org/toc/1421-9778Test
DOI: 10.1159/000373930
الوصول الحر: https://doaj.org/article/eeda18d13cf64b3f83039a039587c149Test
رقم الانضمام: edsdoj.18d13cf64b3f83039a039587c149
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:10158987
14219778
DOI:10.1159/000373930