Protective Effects of Millettia Pulchra Flavonoids on Myocardial Ischemia In Vitro and In Vivo
| العنوان: | Protective Effects of Millettia Pulchra Flavonoids on Myocardial Ischemia In Vitro and In Vivo |
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| المؤلفون: | Xiaoqun Duan, Li Yingxin, Jian Chen, Feizhang Qin, Jie Jian, Renbin Huang, Jianchun Huang, Xudong Zhang, Yongwen Li |
| المصدر: | Cellular Physiology and Biochemistry, Vol 35, Iss 2, Pp 516-528 (2015) |
| بيانات النشر: | Cell Physiol Biochem Press GmbH & Co KG, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, Myocardial ischemia, Physiology, Nitric Oxide Synthase Type II, Apoptosis, Pharmacology, Biology, In Vitro Techniques, Antioxidants, Millettia, lcsh:Physiology, Superoxide dismutase, Rats, Sprague-Dawley, lcsh:Biochemistry, chemistry.chemical_compound, In vivo, Lactate dehydrogenase, medicine, Animals, lcsh:QD415-436, Cells, Cultured, chemistry.chemical_classification, Flavonoids, Dose-Response Relationship, Drug, L-Lactate Dehydrogenase, lcsh:QP1-981, Glutathione peroxidase, Millettia pulchra flavonoids, medicine.disease, Malondialdehyde, Rats, Nitric oxide synthase, Disease Models, Animal, Biochemistry, chemistry, Gene Expression Regulation, Cardiac hemodynamics, biology.protein, Female, Reperfusion injury |
| الوصف: | Background: Previous studies have demonstrated that Millettia pulchra flavonoids (MPF) exhibit protective effects on myocardial ischemia reperfusion injury (MI/RI) in isolated rat hearts and show anti-oxidative, anti-hypoxic and anti-stress properties. Methods: In this study, the cardioprotective effects of MPF on myocardial ischemia and its underlying mechanisms were investigated by a hypoxia/ reoxygenation (H/R) injury model in vitro and a rat MI/RI model in vivo. Results: We found that the lactate dehydrogenase (LDH) and inducible nitric oxide synthase (iNOS) activities were decreased in the MPF pretreatment group, whereas the activities of constructional nitric oxide synthase (cNOS), total nitric oxide synthase (tNOS), Na+-K+-ATPase and Ca2+-Mg2+-ATPase were significantly increased. In addition, the cardiocytes were denser in the MPF groups than in the control group. The mortality rate and apoptosis rate of cardiocytes were significantly decreased. Furthermore, pretreatment with MPF in vivo significantly improved the hemodynamics, decreased malondialdehyde (MDA) abundance, increased the activities of plasma superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and decreased the expression of the Bax protein and ratio Bax/Bc1-2 ration. Conclusions: These results suggest that MPF is an attractive protective substance in myocardial ischemia due to its negative effects on heart rate and ionotropy, reduction of myocardial oxidative damage and modulation of gene expression associated with apoptosis. |
| اللغة: | English |
| تدمد: | 1421-9778 1015-8987 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1a6cf89066ce50c1a8350f29df314d6Test http://www.karger.com/Article/FullText/369716Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e1a6cf89066ce50c1a8350f29df314d6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14219778 10158987 |
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