دورية أكاديمية

QsvR and OpaR coordinately regulate the transcription of cpsS and cpsR in Vibrio parahaemolyticus

التفاصيل البيبلوغرافية
العنوان: QsvR and OpaR coordinately regulate the transcription of cpsS and cpsR in Vibrio parahaemolyticus
المؤلفون: Li, Xue, Lian, Wei, Zhang, Miaomiao, Luo, Xi, Zhang, Yiquan, Lu, Renfei
المساهمون: Research Projects of Nantong Health Commission
المصدر: Canadian Journal of Microbiology ; volume 70, issue 4, page 128-134 ; ISSN 0008-4166 1480-3275
بيانات النشر: Canadian Science Publishing
سنة النشر: 2024
مصطلحات موضوعية: Genetics, Molecular Biology, Applied Microbiology and Biotechnology, General Medicine, Immunology, Microbiology
الوصف: Vibrio parahaemolyticus, the leading cause of seafood-associated gastroenteritis, has a strong capacity to form biofilms on surfaces, which is strictly regulated by the CpsS–CpsR–CpsQ regulatory cascade. OpaR, a master regulator of quorum sensing, is a global regulator that controls multiple cellular pathways including biofilm formation and virulence. QsvR is an AraC-type regulator that works coordinately with OpaR to control biofilm formation and virulence gene expression of V. parahaemolyticus. QsvR and OpaR activate cpsQ transcription. OpaR also activates cpsR transcription, but lacks the detailed regulatory mechanisms. Furthermore, it is still unknown whether QsvR regulates cpsR transcription, as well as whether QsvR and OpaR regulate cpsS transcription. In this study, the results of quantitative real-time PCR and LacZ fusion assays demonstrated that deletion of qsvR and/or opaR significantly decreased the expression levels of cpsS and cpsR compared to the wild-type strain. However, the results of two-plasmid lacZ reporter and electrophoretic mobility-shift assays showed that both QsvR and OpaR were unable to bind the regulatory DNA regions of cpsS and cpsR. Therefore, transcription of cpsS and cpsR was coordinately and indirectly activated by QsvR and OpaR. This work enriched our knowledge on the regulatory network of biofilm formation in V. parahaemolyticus.
نوع الوثيقة: article in journal/newspaper
اللغة: English
DOI: 10.1139/cjm-2023-0196
الإتاحة: https://doi.org/10.1139/cjm-2023-0196Test
حقوق: http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMiningTest
رقم الانضمام: edsbas.14BCC55F
قاعدة البيانات: BASE