Multiple metabolic pathways for metabolism of l -tryptophan in Fusarium graminearum
| العنوان: | Multiple metabolic pathways for metabolism of |
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| المؤلفون: | Anne Johnston, Huiyan Zhao, Linda J. Harris, Caro-Lyne DesRoches, Kun Luo, Thérèse Ouellet |
| المصدر: | Canadian Journal of Microbiology. 63:921-927 |
| بيانات النشر: | Canadian Science Publishing, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Fusarium, Indoles, Immunology, Applied Microbiology and Biotechnology, Microbiology, 03 medical and health sciences, Head blight, Botany, Genetics, Molecular Biology, Pathogen, Triticum, Indoleacetic Acids, biology, Gene Expression Profiling, Tryptophan, food and beverages, General Medicine, Metabolism, Microarray Analysis, biology.organism_classification, Cereal grain, Metabolic pathway, 030104 developmental biology, Edible Grain, Trichothecenes, Metabolic Networks and Pathways |
| الوصف: | Fusarium graminearum is a plant pathogen that can cause the devastating cereal grain disease fusarium head blight in temperate regions of the world. Previous studies have shown that F. graminearum can synthetize indole-3-acetic acid (auxin) using l-tryptophan (L-TRP)-dependent pathways. In the present study, we have taken a broader approach to examine the metabolism of L-TRP in F. graminearum liquid culture. Our results showed that F. graminearum was able to transiently produce the indole tryptophol when supplied with L-TRP. Comparative gene expression profiling between L-TRP-treated and control cultures showed that L-TRP treatment induced the upregulation of a series of genes with predicted function in the metabolism of L-TRP via anthranilic acid and catechol towards the tricarboxylic acid cycle. It is proposed that this metabolic activity provides extra energy for 15-acetyldeoxynivalenol production, as observed in our experiments. This is the first report of the use of L-TRP to increase energy resources in a Fusarium species. |
| تدمد: | 1480-3275 0008-4166 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c73c2487f61dd9f954dd41ff67d1b2c0Test https://doi.org/10.1139/cjm-2017-0383Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c73c2487f61dd9f954dd41ff67d1b2c0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14803275 00084166 |
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