A comparison of the electrophoretic properties of dehydrogenases from Arthrobacter, Agrobacterium, and Rhizobium
| العنوان: | A comparison of the electrophoretic properties of dehydrogenases from Arthrobacter, Agrobacterium, and Rhizobium |
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| المؤلفون: | J. W. Rouatt, Vesna Purkayastha, G. W. Skyring |
| المصدر: | Canadian Journal of Microbiology. 17:961-966 |
| بيانات النشر: | Canadian Science Publishing, 1971. |
| سنة النشر: | 1971 |
| مصطلحات موضوعية: | Agrobacterium, Immunology, Alcohol oxidoreductase, Glucosephosphate Dehydrogenase, Applied Microbiology and Biotechnology, Microbiology, Malate dehydrogenase, Glutamate Dehydrogenase, Species Specificity, Malate Dehydrogenase, Arthrobacter, Genetics, Phosphogluconate dehydrogenase, Molecular Biology, Orotic Acid, L-Lactate Dehydrogenase, Staining and Labeling, biology, Phosphogluconate Dehydrogenase, Succinate dehydrogenase, Glutamate dehydrogenase, General Medicine, Electrophoresis, Disc, biology.organism_classification, Isocitrate Dehydrogenase, Succinate Dehydrogenase, Alcohol Oxidoreductases, Biochemistry, biology.protein, Rhizobium, Indicators and Reagents |
| الوصف: | Eleven Arthrobacter, three Agrobacterium, and three Rhizobium cultures were characterized in terms of the electrophoretic properties of dehydrogenases active upon lactate, succinate, malate, glucose-6-phosphate, 6-phosphogluconate, isocitrate, glutamate, and dihydroorotate (K). Comparisons of the zymograms for each organism were made on the basis of selected data. Overall similarities so found corresponded in general with groupings of organisms found previously. A high degree of affinity between the arthrobacters and the rhizobia–agrobacteria seemed unlikely, but the results for glutamic dehydrogenase, dihydroorotate dehydrogenase, and to some extent those for lactic and malic dehydrogenases suggested that there may be some affinity not previously suspected.We have concluded that while dehydrogenase zymograms may be useful for the characterization of microorganisms, their application in bacterial taxonomy is not simple. Original or even edited data would not be appropriate for simple kinds of computer-assisted analysis without means of determining the extent of homology existing between enzymes from different sources. |
| تدمد: | 1480-3275 0008-4166 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70cfd31fd9c1693827da880aa229f97cTest https://doi.org/10.1139/m71-153Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....70cfd31fd9c1693827da880aa229f97c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14803275 00084166 |
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