The in vitro effect of Actinobacillus actinomycetemcomitans strain Y4 lipopolysaccharide on murine peritoneal macrophages
| العنوان: | The in vitro effect of Actinobacillus actinomycetemcomitans strain Y4 lipopolysaccharide on murine peritoneal macrophages |
|---|---|
| المؤلفون: | S. C. Holt, Kathleen A. Barker |
| المصدر: | Canadian Journal of Microbiology. 29:1552-1563 |
| بيانات النشر: | Canadian Science Publishing, 1983. |
| سنة النشر: | 1983 |
| مصطلحات موضوعية: | Lipopolysaccharides, Programmed cell death, Lipopolysaccharide, Cell Survival, Acid Phosphatase, Immunology, Cell, Mice, Inbred Strains, Biology, Applied Microbiology and Biotechnology, Microbiology, Mice, chemistry.chemical_compound, Lactate dehydrogenase, Genetics, medicine, Animals, Macrophage, Pseudopodia, Molecular Biology, L-Lactate Dehydrogenase, Macrophages, Actinobacillus, General Medicine, biology.organism_classification, Organoids, Microscopy, Electron, medicine.anatomical_structure, chemistry, Microscopy, Electron, Scanning, lipids (amino acids, peptides, and proteins), Peritoneum, Lysosomes, Cell activation, Intracellular |
| الوصف: | Actinobacillus actinomycetemcomitans strain Y4 lipopolysaccharide (LPS) was examined for its in vitro effect on murine peritoneal macrophage morphology, viability, and lysosomal enzyme activity. Fifty micrograms of Y4 LPS per 106 macrophages resulted in macrophage activation, eliciting the release of acid phosphates (AcP), as well as the accumulation of intracellular AcP, without a loss in viability. There was also an increase in the number of organelles and cell-surface ruffles. One hundred or 250 μg of Y4 LPS caused flattening, rounding, and blebbing of the cell surface, as well as the release of large quantities of AcP and lactate dehydrogenase (LDH), within 5 min of exposure to the LPS. Alteration of cell ultrastructure occurred within 30 min, and extensive autophagocytosis by 24 h, indicative of cell injury. Short-term (less than 24 h) experiments appear necessary to distinguish between the effects of cell activation and cell death caused by A. actinomycetemcomitans Y4 LPS. |
| تدمد: | 1480-3275 0008-4166 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b2e544c433a3d2ccf51d998bc5a417cTest https://doi.org/10.1139/m83-238Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....2b2e544c433a3d2ccf51d998bc5a417c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14803275 00084166 |
|---|