دورية أكاديمية
3439 Role of the Airway Microbiome in Viral Bronchiolitis Associated Respiratory Failure
| العنوان: | 3439 Role of the Airway Microbiome in Viral Bronchiolitis Associated Respiratory Failure |
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| المؤلفون: | Wasserman, Emily, Worgall, Stefan, Sharma, Anurag |
| المصدر: | Journal of Clinical and Translational Science ; volume 3, issue s1, page 20-20 ; ISSN 2059-8661 |
| بيانات النشر: | Cambridge University Press (CUP) |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | General Medicine |
| الوصف: | OBJECTIVES/SPECIFIC AIMS: This study aims to determine if a bronchiolitis specific microbiome exists and how it evolves through disease course. Objective 1. Determine the microbiome profile of the airway in virus induced bronchiolitis-associated respiratory failure. Objective 2. Identify changes in the airway microbiome profile through the course of virus induced bronchiolitis associated respiratory failure, and the relationship between microbiome composition and clinical respiratory status. Objective 3. Determine the impact of rhinovirus infection on the lung and stool microbiome in a murine asthma model. METHODS/STUDY POPULATION: Objectives 1 &2: We are conducting a single-center prospective case-control study of patients admitted to the Komansky - Weill Cornell Pediatric Critical Care Unit. Infants less than two years of age with a diagnosis of bronchiolitis requiring intubation and mechanical ventilation are enrolled as subjects. Infants less than two years of age intubated and requiring mechanical ventilation without primary lung pathology are enrolled as controls. To evaluate our primary objective, tracheal aspirates will be collected from both subjects and controls on the day of intubation. We will perform 16s RNA sequencing on the tracheal aspirate samples and compare the resulting microbiomes. To evaluate secondary objective, we will collect tracheal aspirates of our study population on a daily basis and map the microbiome in parallel with objective measures of respiratory status including oxygen index and successful extubation. Both subjects and controls are being enrolled as a convenience sample. Objective 3: Mice, heterozygous for the sptlc2 gene (Spltc2 +/−) demonstrate reduced de-novo sphingolipids and increased airway hyperresponsiveness with methacholine challenge. Airway hyper-responsiveness is a cardinal feature of asthma. This airway hyperresponsiveness is exacerbated in the setting of rhinovirus (Figure 1). Using 16s sequencing, we will examine the lung microbiome of Sptlc2 +/− ad Sptlc2 ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1017/cts.2019.50 |
| الإتاحة: | https://doi.org/10.1017/cts.2019.50Test https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S2059866119000505Test |
| حقوق: | http://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: | edsbas.A07087C5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1017/cts.2019.50 |
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