رسالة جامعية
Antimicrobial Resistance in Foot rot Bacteria
| العنوان: | Antimicrobial Resistance in Foot rot Bacteria |
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| المؤلفون: | Kim, Eunjee |
| المساهمون: | Morck, Douglas |
| بيانات النشر: | Graduate Studies University of Calgary Calgary |
| سنة النشر: | 2014 |
| المجموعة: | PRISM - University of Calgary Digital Repository |
| مصطلحات موضوعية: | Microbiology, Biology--Molecular, Bovine foot rot, Tetracycline resistance, Erythromycin resistance, Fusobacterium necrophorum, Porphyromonas levii, Prevotella melaninogenica, antimicrobial resistance, Anaerobe |
| الوصف: | This study set out to determine tetracycline and erythromycin resistance profiles and detect various tetracycline (tetB, tetC, tetL, tetM, tetQ and tetW) or erythromycin (ermA, ermB and ermF) resistance determinants from clinical isolates of bovine foot rot bacteria isolated from affected animals in Alberta. Using agar dilution assay, we determined that 82% (32/39) of Fusobacterium necrophorum were tetracycline resistant and 62% (24/39) were classified intermediate to erythromycin. Approximately 82% (18/22) and 91% (20/22) of Prevotella melaninogenica was sensitive to tetracycline and erythromycin respectively, For Porphyromonas levii, 88% (21/24) were sensitive to tetracycline and 100% (24/24) were erythromycin sensitive. Polymerase chain reaction amplification revealed that tetC, tetL, tetM and ermF were commonly detected in F.necrophorum, and tetQ in Pr.melaninogenica and P.levii. Special resistance determinant patterns were not observed in antibiotic resistant strains compared to sensitive isolates in all three pathogens because as high as 90% of resistance determinants were detected in both resistant and sensitive strains of bacteria. |
| نوع الوثيقة: | master thesis |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | Kim, E. (2014). Antimicrobial Resistance in Foot rot Bacteria (Unpublished master's thesis). University of Calgary, Calgary, AB. doi:10.11575/PRISM/24937; http://dx.doi.org/10.11575/PRISM/24937Test; http://hdl.handle.net/11023/1544Test |
| DOI: | 10.11575/PRISM/24937 |
| الإتاحة: | https://doi.org/10.11575/PRISM/24937Test http://hdl.handle.net/11023/1544Test |
| حقوق: | University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. |
| رقم الانضمام: | edsbas.5347367D |
| قاعدة البيانات: | BASE |
| DOI: | 10.11575/PRISM/24937 |
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