مورد إلكتروني
The Roles of RasGAP SH3 Domain Binding Proteins (G3BPs) in RNA Metabolism, the Cellular Stress Response and Tumorigenesis
| العنوان: | The Roles of RasGAP SH3 Domain Binding Proteins (G3BPs) in RNA Metabolism, the Cellular Stress Response and Tumorigenesis |
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| بيانات النشر: | Griffith University Brisbane 2006 |
| تفاصيل مُضافة: | Kennedy, Derek Tonissen, Kathryn Stirling, Susan Renee |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Full Text Thesis (PhD Doctorate) Doctor of Philosophy (PhD) School of Biomolecular and Biomedical Sciences G3BP1 and G3BP2 are members of a highly conserved family of multi-functional RNA binding proteins, which appear to co-ordinate signal transduction and post-transcriptional gene regulation. Both proteins are over-expressed in cancer, and G3BP1 promotes cell proliferation and survival. Aberrant expression of various RNA binding proteins is common in cancer, and several of these proteins influence tumorigenesis. Therefore, detailed examination of RNA binding proteins, such as G3BPs, may provide insights into the post-transcriptional mechanisms underlying tumorigenesis. Tumours arise as a consequence of genetic mutation or alteration, which often result from stress-induced DNA damage. Cancer progression is facilitated by various epigenetic stress adaptation mechanisms. Stressful stimuli induce transitory translational shut-off, mediated by phosphorylation of eukaryotic initiation factor alpha;(eIF2alpha;). This phosphorylation event leads to formation of discrete cytoplasmic foci known as stress granules (SGs), which are translationally-silent sites of mRNA sorting. It was initially thought that an RNA-binding protein, T-cell internal antigen 1 (TIA-1), was instrumental in both the formation and functioning of SGs, because over-expression of TIA-1 induces spontaneous SGs and concomitantly causes a decrease in reporter gene expression. It is now clear that SG content can change depending on the type of stress, and that various proteins, including G3BP1, can induce spontaneous SGs. In vitro evidence previously implicated both G3BP1 and G3BP2 as endoribonucleases, so it was suggested that G3BPs act to target mRNA for decay at the SG. This project sought to further investigate this proposal, and in this way gain insight into the specific function of G3BPs in post-transcriptional regulation during tumorigenesis. Characterisation of G3BP1 and G3BP2 expression and localisation patterns in human cells and cancer was necessary before functional analyses in human cell systems cou |
| مصطلحات الفهرس: | RasGAP SH3 domain binding proteins, RNA metabolism, G3BP1 expression, G3BP2 expression, tumours, cancer, tumorigenesis, Griffith thesis |
| الإتاحة: | Open access content. Open access content The author owns the copyright in this thesis, unless stated otherwise. Public The author owns the copyright in this thesis, unless stated otherwise. |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | LG0 oai:research-repository.griffith.edu.au:10072/366889 10.25904/1912/2645 1327828653 |
| المصدر المساهم: | GRIFFITH UNIV From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1327828653 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |