دورية أكاديمية
Coxsackie-adenovirus receptor expression is enhanced in pancreas from patients with type 1 diabetes
| العنوان: | Coxsackie-adenovirus receptor expression is enhanced in pancreas from patients with type 1 diabetes |
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| المؤلفون: | Hodik, M., Anagandula, M., Fuxe, J., Krogvold, L., Dahl Jørgensen, K., Hyã¶ty, H., Sarmiento, L., Frisk, G, Atkinson, Mark, Pugliese, Alberto, Campbell Thompson, Martha, Kaddis, John, Kusmartseva, Irina, Yang, Mingder, Clare Salzler, Michael, Chapman, Nora, Tracy, Steven, Smithee, Shane, Alhazmi, Abdulaziz, Frisk, Gun, Anagandula, Mahesh, Sarimiento, Luis, Giepmans, Ben, Kalicharan, Ruby, Kuipers, Jeroen, Wolters, Anouk, Jelmer, Willems, Morgan, Noel, Richardson, Sarah, Dyahal, Shalinee, Russell, Mark, Leete, Pia, Nadler, Jerry, Morris, Margaret, Nyalwidhe, Julius, Burch, Tayna, von Herrath, Matthias, Rodriguez Calvo, Teresa, Sabouri, Somayeh, Anquetil Besnard, Florence, Plagnol, Vincent, Breuer, Judith, Depledge, Daniel, Dotta, Francesco, Sebastiani, Guido, Patti, Aurora, Nigi, Laura, Ventriglia, Giuliana, Mancarella, Francesca, Gerling, Ivan, Kakoola, Dorothy, Lenchik, Nataliya, Hyã¶ty, Heikki, Laiho, Jutta, Oikarnen, Maarit, Eizirik, Decio, Opdebeeck, Anne, Lloyd, Richard, Petrosino, Joseph, Homann, Dirk, Kent, Sally, Sarkar, Suparna, Toniolo, Antonio, Gianani, Roberto, Horwitz, Marc, Marjomaki, Varpu, Flodström Tullberg, Malin, Domsgen, Erna, Larsson, Pã¤r, PIEMONTI, LORENZO |
| المساهمون: | Hodik, M., Anagandula, M., Fuxe, J., Krogvold, L., Dahl Jørgensen, K., Hyã¶ty, H., Sarmiento, L., Frisk, G, Atkinson, Mark, Pugliese, Alberto, Campbell Thompson, Martha, Kaddis, John, Kusmartseva, Irina, Yang, Mingder, Clare Salzler, Michael, Chapman, Nora, Tracy, Steven, Smithee, Shane, Alhazmi, Abdulaziz, Frisk, Gun, Anagandula, Mahesh, Sarimiento, Lui, Giepmans, Ben, Kalicharan, Ruby, Kuipers, Jeroen, Wolters, Anouk, Jelmer, Willem, Morgan, Noel, Richardson, Sarah, Dyahal, Shalinee, Russell, Mark, Leete, Pia, Nadler, Jerry, Morris, Margaret, Nyalwidhe, Juliu, Burch, Tayna, von Herrath, Matthia, Rodriguez Calvo, Teresa, Sabouri, Somayeh, Anquetil Besnard, Florence, Plagnol, Vincent, Breuer, Judith, Depledge, Daniel, Dotta, Francesco, Sebastiani, Guido, Patti, Aurora, Nigi, Laura, Ventriglia, Giuliana, Mancarella, Francesca, Gerling, Ivan, Kakoola, Dorothy, Lenchik, Nataliya, Hyã¶ty, Heikki, Laiho, Jutta, Oikarnen, Maarit, Eizirik, Decio, Opdebeeck, Anne, Lloyd, Richard, Petrosino, Joseph, Homann, Dirk, Kent, Sally, Sarkar, Suparna, Toniolo, Antonio, Gianani, Roberto, Piemonti, Lorenzo, Horwitz, Marc, Marjomaki, Varpu, Flodström Tullberg, Malin, Domsgen, Erna, Larsson, Pã¤r |
| بيانات النشر: | BMJ Publishing Group |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Islet of Langerhan, Receptor(s), Type 1, Viru, Endocrinology, Diabetes and Metabolism |
| الوصف: | Objectives: One of the theories connecting enterovirus (EV) infection of human islets with type 1 diabetes (T1D) is the development of a fertile field in the islets. This implies induction of appropriate proteins for the viral replication such as the coxsackie- adenovirus receptor (CAR). The aim of this study was to investigate to what extent CAR is expressed in human islets of Langerhans, and what conditions that would change the expression. Design: Immunohistochemistry for CAR was performed on paraffin-embedded pancreatic tissue from patients with T1D (n=9 recent onset T1D, n=4 long-standing T1D), islet autoantibody-positive individuals (n=14) and non-diabetic controls (n=24) individuals. The expression of CAR was also examined by reverse transcription PCR on microdissected islets (n=5), exocrine tissue (n=5) and on explanted islets infected with EV or exposed to chemokines produced by EV-infected islet cells. Results: An increased frequency of patients with T1D and autoantibody-positive individuals expressed CAR in the pancreas (p<0.039). CAR staining was detected more frequently in pancreatic islets from patients with T1D and autoantibody-positive subjects (15/27) compared with (6/24) non-diabetic controls (p<0.033). Also in explanted islets cultured in UV-treated culture medium from coxsackievirus B (CBV)-1-infected islets, the expression of the CAR gene was increased compared with controls. Laser microdissection of pancreatic tissue revealed that CAR expression was 10-fold higher in endocrine compared with exocrine cells of the pancreas. CAR was also expressed in explanted islets and the expression level decreased with time in culture. CBV-1 infection of explanted islets clearly decreased the expression of CAR (p<0.05). In contrast, infection with echovirus 6 did not affect the expression of CAR. Conclusions: CAR is expressed in pancreatic islets of patients with T1D and the expression level of CAR is increased in explanted islets exposed to proinflammatory cytokines/chemokines produced by ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/WOS:000506148900001; volume:4; issue:1; firstpage:e000219; journal:BMJ OPEN DIABETES RESEARCH AND CARE; http://hdl.handle.net/20.500.11768/60524Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84998774606; http://drc.bmj.comTest/ |
| DOI: | 10.1136/bmjdrc-2016-000219 |
| الإتاحة: | https://doi.org/20.500.11768/60524Test https://doi.org/10.1136/bmjdrc-2016-000219Test https://hdl.handle.net/20.500.11768/60524Test http://drc.bmj.comTest/ |
| رقم الانضمام: | edsbas.4AE63156 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1136/bmjdrc-2016-000219 |
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