Chemotaxis of Vδ2 T cells to the joints contributes to the pathogenesis of rheumatoid arthritis
| العنوان: | Chemotaxis of Vδ2 T cells to the joints contributes to the pathogenesis of rheumatoid arthritis |
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| المؤلفون: | Shan-Shan Yin, Lidan Zhao, Wei He, Yujia Mao, Yunyun Fei, Jianmin Zhang, Xuan Zhang, Hua Chen, Wenjie Zheng, Jing-bo Guo, Wen-Xiu Mo, Huaxia Yang, Chen Zhou, Jiaxin Zhou, Wen Zhang, Linfang Huang |
| المصدر: | Annals of the Rheumatic Diseases |
| بيانات النشر: | BMJ Publishing Group, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, rheumatoid arthritis, Receptors, CXCR3, Receptors, CCR5, T-Lymphocytes, Immunology, Population, T cells, CXCR3, General Biochemistry, Genetics and Molecular Biology, Proinflammatory cytokine, Arthritis, Rheumatoid, 03 medical and health sciences, Chemokine receptor, Interleukin 21, 0302 clinical medicine, Rheumatology, Cell Movement, Osteoarthritis, medicine, Humans, Immunology and Allergy, education, Basic and Translational Research, education.field_of_study, Interleukin-6, Tumor Necrosis Factor-alpha, business.industry, Chemotaxis, Synovial Membrane, Interleukin, Receptors, Antigen, T-Cell, gamma-delta, anti-TNF, Flow Cytometry, medicine.disease, 030104 developmental biology, Case-Control Studies, Rheumatoid arthritis, Tumor necrosis factor alpha, business, 030215 immunology |
| الوصف: | ObjectivesTo explore the role of Vδ2 T cells in the pathogenesis of rheumatoid arthritis (RA).MethodsSixty-eight patients with RA, 21 patients with osteoarthritis and 21 healthy controls were enrolled in the study. All patients with RA fulfilled the 2010 American College of Rheumatology/European League Against Rheumatism criteria for RA. Peripheral Vδ2T population, chemokine receptor expression and proinflammatory cytokine secretion were quantified by flow cytometry. The infiltration of Vδ2 T cells within the synovium was examined by immunohistochemistry and flow cytometry. The effect of tumour necrosis factor (TNF)-α and interleukin (IL)-6 on Vδ2 T migration was determined by flow cytometry and transwell migration assay.ResultsPeripheral Vδ2T cells, but not Vδ1 T cells, were significantly lower in patients with RA, which was negatively correlated with disease activity gauged by Disease Activity Score in 28 joints. Vδ2 T cells from RA accumulated in the synovium and produced high levels of proinflammatory cytokines including interferon-γ and IL-17. Phenotypically, Vδ2 T cells from RA showed elevated chemotaxis potential and expressed high levels of chemokine receptors CCR5 and CXCR3, which was driven by increased serum TNF-α through nuclear factor kappa B signalling. In vivo, TNF-α neutralising therapy dramatically downregulated CCR5 and CXCR3 on Vδ2 T cells and repopulated the peripheral Vδ2 T cells in patients with RA.ConclusionsHigh levels of TNF-α promoted CCR5 and CXCR3 expression in Vδ2 T cells from RA, which potentially infiltrated into the synovium and played crucial roles in the pathogenesis of RA. Targeting Vδ2 T cells might be a potential approach for RA. |
| اللغة: | English |
| تدمد: | 1468-2060 0003-4967 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dcedff7ca177d148de96d2675e4c978aTest http://europepmc.org/articles/PMC5705844Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dcedff7ca177d148de96d2675e4c978a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14682060 00034967 |
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