Interaction between tumour-infiltrating B cells and T cells controls the progression of hepatocellular carcinoma
| العنوان: | Interaction between tumour-infiltrating B cells and T cells controls the progression of hepatocellular carcinoma |
|---|---|
| المؤلفون: | Kiat Hon Lim, Alexander Y. F. Chung, Alessandra Nardin, Valerie Chew, Chun Jye Lim, Jean-Pierre Abastado, Irene Oi-Lin Ng, Han Chong Toh, Jinmiao Chen, Qingfeng Chen, Alex Tan, Achim Weber, Mathias Heikenwalder, Marta Garnelo, Pierce K. H. Chow, London Lucien Pj Ooi, Zhisheng Her, Joe Yeong |
| المساهمون: | University of Zurich, Chew, Valerie |
| المصدر: | Gut 66, 342-351 (2015) Gut |
| بيانات النشر: | Bmj Publishing Group, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Pathology, CD3 Complex, T-Lymphocytes, Gene Expression, CD38, Granzymes, Interleukin 21, Mice, 0302 clinical medicine, Aged, 80 and over, B-Lymphocytes, biology, Caspase 3, Liver Neoplasms, Gastroenterology, Middle Aged, Survival Rate, medicine.anatomical_structure, Phenotype, 030220 oncology & carcinogenesis, Hepatocellular carcinoma, CANCER IMMUNOBIOLOGY, B Cell, Cancer Immunobiology, Hepatocellular Carcinoma, Immunoregulation, T Lymphocytes, Disease Progression, Female, Adult, medicine.medical_specialty, Carcinoma, Hepatocellular, T cell, CD8 Antigens, Antigens, CD19, 610 Medicine & health, CD19, Lymphocyte Depletion, B CELL, 03 medical and health sciences, Interferon-gamma, Young Adult, Lymphocytes, Tumor-Infiltrating, Antigens, CD, 10049 Institute of Pathology and Molecular Pathology, medicine, Animals, Humans, 2715 Gastroenterology, HEPATOCELLULAR CARCINOMA, CD40 Antigens, B cell, Aged, Retrospective Studies, IMMUNOREGULATION, CD40, Hepatology, business.industry, medicine.disease, Antigens, CD20, ADP-ribosyl Cyclase 1, Tumor Necrosis Factor Receptor Superfamily, Member 7, Granzyme B, Mice, Inbred C57BL, 030104 developmental biology, Ki-67 Antigen, Cancer research, biology.protein, T LYMPHOCYTES, business |
| الوصف: | OBJECTIVE: The nature of the tumour-infiltrating leucocytes (TILs) is known to impact clinical outcome in carcinomas, including hepatocellular carcinoma (HCC). However, the role of tumour-infiltrating B cells (TIBs) remains controversial. Here, we investigate the impact of TIBs and their interaction with T cells on HCC patient prognosis. DESIGN: Tissue samples were obtained from 112 patients with HCC from Singapore, Hong Kong and Zurich and analysed using immunohistochemistry and immunofluorescence. RNA expression of CD19, CD8A, IFNG was analysed using quantitative PCR. The phenotype of freshly isolated TILs was analysed using flow cytometry. A mouse model depleted of mature B cells was used for functional study. RESULTS: Tumour-infiltrating T cells and B cells were observed in close contact with each other and their densities are correlated with superior survival in patients with HCC. Furthermore, the density of TIBs was correlated with an enhanced expression of granzyme B and IFN-γ, as well as with reduced tumour viability defined by low expression of Ki-67, and an enhanced expression of activated caspase-3 on tumour cells. CD27 and CD40 costimulatory molecules and TILs expressing activation marker CD38 in the tumour were also correlated with patient survival. Mice depleted of mature B cells and transplanted with murine hepatoma cells showed reduced tumour control and decreased local T cell activation, further indicating the important role of B cells. CONCLUSIONS: The close proximity of tumour-infiltrating T cells and B cells indicates a functional interaction between them that is linked to an enhanced local immune activation and contributes to better prognosis for patients with HCC. |
| وصف الملف: | application/pdf; Garnelo_Gut_2015.pdf - application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c54eb0e8cafe98ef5b66810444f7bbbeTest https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=47551Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c54eb0e8cafe98ef5b66810444f7bbbe |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |