Objective To determine if Mycobacterium tuberculosis can be detected in corneas of donors who have active tuberculosis (TB) disease using polymerase chain reaction (PCR) or culture. Design This is a prospective cross-sectional study. Participants 25 corneas (12 from TB-negative donors and 13 from TB-positive donors) from the Sta. Lucia International Eye Bank were submitted to the TB Research Laboratory, Medical Research Laboratory, Department of Medicine, UP-PGH, for diagnostic evaluation. Intervention Corneas were evaluated using PCR test for M tuberculosis . Acid-fast bacilli smear and culture were also done. Main outcome measures Result of laboratory findings. Results Among 12 TB-negative donors, there were 2 (16.67%) corneas that were PCR negative for M tuberculosis , whereas 10 (83.33%) corneas were PCR positive for M tuberculosis . Among 13 TB-positive donors, there were 7 (53.85%) corneas that were PCR negative for M tuberculosis , whereas 6 (46.15%) corneas were PCR positive for M tuberculosis . All cultures were negative. Conclusion PCR for M tuberculosis was positive for some TB-positive donors as well as TB-negative donors. However, all cultures were negative. It is recommended that further studies be done to investigate if recipients with PCR-positive corneas will eventually lead to disease transmission or not. It is further recommended that such findings be used to re-evaluate criteria for suitability of donors with tuberculosis.
