Detection of epidermal growth factor receptor gene mutations in cytology specimens from patients with non-small cell lung cancer utilising high-resolution melting amplicon analysis
| العنوان: | Detection of epidermal growth factor receptor gene mutations in cytology specimens from patients with non-small cell lung cancer utilising high-resolution melting amplicon analysis |
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| المؤلفون: | Barbara Chadwick, Joel S. Bentz, G D Smith, Carlynn Willmore-Payne |
| المصدر: | Journal of Clinical Pathology. 61:487-493 |
| بيانات النشر: | BMJ, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Male, Pathology, medicine.medical_specialty, Lung Neoplasms, Biopsy, Fine-Needle, DNA Mutational Analysis, Molecular Sequence Data, Tissue Banks, Biology, Gene mutation, Polymerase Chain Reaction, High Resolution Melt, Pathology and Forensic Medicine, Carcinoma, Non-Small-Cell Lung, medicine, Humans, Amino Acid Sequence, Epidermal growth factor receptor, Lung cancer, Aged, Retrospective Studies, EGFR inhibitors, Aged, 80 and over, Cancer, DNA, Neoplasm, Genes, erbB-1, General Medicine, Middle Aged, medicine.disease, Real-time polymerase chain reaction, Mutation, Cancer research, biology.protein, Adenocarcinoma, Female |
| الوصف: | Background:Activating epidermal growth factor receptor (EGFR) mutations have been implicated in non-small cell lung cancer (NSCLC), and have also been clinically correlated with patient sensitivity to targeted EGFR inhibitors.Aim:To describe a technique for determining EGFR mutation status on archival fine needle aspirate (FNA) specimens from advanced NSCLC patients.Methods:Eleven archival FNA slides from patients with advanced NSCLC were examined for diagnostic material to identify tumour cell-enriched regions. EGFR mutation status was determined using a slide-scrape DNA extraction protocol of selected tumour cell regions on the smear slides, followed by real time PCR and high resolution melt analysis (HRMAA) of EGFR exons 18, 19, 20, and 21, followed by sequence analysis.Results:All DNA samples were successfully amplified by PCR. Three adenocarcinoma patient samples contained EGFR mutations in exon 19 (L747-P753insS). One of the three had an additional exon 19 mutation (A755D).Conclusions:Archival cytology slides from patients with NSCLC can be used to determine EGFR mutation status by PCR, HRMAA, and sequencing. The ability to use archival cytology slides greatly increases the potential material available for molecular analysis in diagnosis and selection of patients for targeted therapeutic agents. |
| تدمد: | 1472-4146 0021-9746 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b7ed4b89c7bb2a8bbe47cdebc8a06d5Test https://doi.org/10.1136/jcp.2007.051425Test |
| رقم الانضمام: | edsair.doi.dedup.....0b7ed4b89c7bb2a8bbe47cdebc8a06d5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14724146 00219746 |
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